IGEM:MIT/2005/Receiver 1: ToxR

Receiver Device #1 -- ToxR

POC

wiki-Will

Function

To dimerize in the presence of the input signal and cause gene expression to turn on.

Device Depiction

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  ToxR Pathway

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  Edit ToxR.ppt

Information path ...

1. Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
2. Fluoroscein binds to adjacent ToxR'-scFv fusions.
   1. Causes ToxR' dimerization
3. GFP expressed @ CTX_promoter
   1. As a function of ToxR dimerization

People path ...

1. Maxine gives us Fluoroscein, or identifies something else
2. Maxine's antigen binds to Jenny's scFv
3. Then, Jenny's scFv induces Will's ToxR' to dimerize
4. The dimerization leads to the expression of Jessica's output @ CTXpromoter [cI, maybe]
5. Ray makes the whole system look like "we meant to do that."
6. Jen makes sure it all works.

Current Status

1. About to order all necassary proteins and primers for first synthesis.
2. Begin design and outline necassary experiments for system.
3. Develop brief yet throrough system explanation.
4. Need to verify that we can get our signal into the periplasm
   1. Recieve olgo's 07/13. LEts throw them in our cells
   2. and if it doesn't "just work," develop and specify how we have to manipulate / weaken our cells to make it work.
   3. lets make it WORK
5. Continue'd research on putting our scFv onto the outermembrane of a cell.

Open Issues/Questions

• !

1. How will we get our initial test antigens into our cell?
2. how will we design a system to find more bigger antigen sets?

1. Can we solve the cell wall permeability problem?