IGEM:MIT/2006/Notebook/2006-8-31: Difference between revisions
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#miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert it at the MCS | #miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert it at the MCS | ||
#analyze incoming sequencing extra well b/c are final bb-box constructs (and throw out duplicates if these all look good) | #analyze incoming sequencing extra well b/c are final bb-box constructs (and throw out duplicates if these all look good) | ||
#pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe | |||
Revision as of 06:33, 30 August 2006
to do
- miniprep iGEM yeast shuttle vector and digest it appropriately (check the MCS, or Veena took notes and might know). Also digest our BGD (J45200,J45220) appropriately to insert it at the MCS
- analyze incoming sequencing extra well b/c are final bb-box constructs (and throw out duplicates if these all look good)
- pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe
