IGEM:MIT/2006/Notebook/2006-8-31: Difference between revisions
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#glycerol (6) J45399 LCs (note: 2XYT problem) '''-done''' and miniprep '''-done''' | #glycerol (6) J45399 LCs (note: 2XYT problem) '''-done''' and miniprep '''-done''' | ||
#primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29) | #primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29) | ||
#check if the 0-Q-199A forward sequencing is in from the re-run '''-done''' (it's all good | #check if the 0-Q-199A forward sequencing is in from the re-run '''-done''' (it's all good) | ||
# | #sequence (6) J45399 attempts (b/c similar # of colonies on 2nd attempt I think its worth a try) '''-done''' | ||
#miniprep iGEM yeast shuttle vector '''-done''' | #miniprep iGEM yeast shuttle vector '''-done''' | ||
#digest yeast with XS. Also digest our ATF1 biobrick (J45014) with XS. '''NOTE:''' we need to do freeze step so that X and S don't religate for both of these!! | #digest yeast with XS. Also digest our ATF1 biobrick (J45014) with XS. '''NOTE:''' we need to do freeze step so that X and S don't religate for both of these!! | ||
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#pick colonies from all 8/30 transformant plates and make LCs | #pick colonies from all 8/30 transformant plates and make LCs | ||
#check on pseudomonas '''-done''' | #check on pseudomonas '''-done''' | ||
==to do tomorrow== | |||
#analyze the incoming 12 sample sequencing order (and throw out duplicates/bad ones) | |||
#sequence J45220-C-mut with internal ATF1 primers- need to order internal primers | |||
==also== | ==also== | ||
#'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | #'''UPDATE REGISTRY''': create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) '''as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!!''' - p.s. anybody out of town is welcome to help. we seem to be very behind on this. | ||
Revision as of 09:53, 31 August 2006
to do
- dilute 4 plate reader LCs 1:500 and load w/Barry at 9 am -done
- glycerol (6) J45399 LCs (note: 2XYT problem) -done and miniprep -done
- primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
- check if the 0-Q-199A forward sequencing is in from the re-run -done (it's all good)
- sequence (6) J45399 attempts (b/c similar # of colonies on 2nd attempt I think its worth a try) -done
- miniprep iGEM yeast shuttle vector -done
- digest yeast with XS. Also digest our ATF1 biobrick (J45014) with XS. NOTE: we need to do freeze step so that X and S don't religate for both of these!!
- ligate ATF1 into yeast shuttle vector and transform into yeast (all in one step, hopefully samantha is around)
- pellet big yeast LC with big centrifuge and resuspend cells in milk and find a good bread recipe for baking
- pick colonies from all 8/30 transformant plates and make LCs
- check on pseudomonas -done
to do tomorrow
- analyze the incoming 12 sample sequencing order (and throw out duplicates/bad ones)
- sequence J45220-C-mut with internal ATF1 primers- need to order internal primers
also
- UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.
