IGEM:MIT/2006/Notebook/2006-8-31

to do

1. dilute 4 plate reader LCs 1:500 and load w/Barry at 9 am
2. glycerol (6) J45399 LCs (note: 2XYT problem)
3. primers should be arriving today, so we should try to PCR pmsCEAB-full out of PE3 and pE3R (minipreps of both plasmids from 8/29)
4. check if the 0-Q-199A forward sequencing is in from the re-run and also analyze the incoming 12 sample sequencing order (and throw out duplicates/bad ones)
5. sequence J45220-C-mut with internal ATF1 primers
6. miniprep iGEM yeast shuttle vector and digest it appropriately. Also digest our ATF1 biobrick (J45014) appropriately to insert it at the MCS
7. ligate ATF1 into yeast shuttle vector and transform into yeast (all in one step)
8. pellet with big centrifuge and resuspend yeast cells in milk and find a good bread recipe for baking
9. pick colonies from all 8/30 transformant plates and make LCs

also

1. UPDATE REGISTRY: create registry biobrick parts and choose numbers for our inverter constructs (w/ and w/out osmY attached) as well as put all intermediate BGD, IAGD, SAGD parts into the registry!!!! - p.s. anybody out of town is welcome to help. we seem to be very behind on this.