IGEM:MIT/2006/Notebook/2006-9-8: Difference between revisions
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#*transform | #*transform | ||
#pmsCEAB | #pmsCEAB | ||
#*digest with ES | #*digest with ES (has 4 Pst sites!!) | ||
#**run results on a gel and clean-up | #**run results on a gel and clean-up | ||
#*ligate into a double resistance backbone:ES (make sure different from w-ever our WGD device is living in) | #*ligate into a double resistance backbone:ES (make sure different from w-ever our WGD device is living in) | ||
#*transform | #*transform | ||
#pellet new yeast cells (pm) and | #pellet new yeast cells (pm) and resuspend in milk -- bake again or bake on saturday | ||
Revision as of 18:49, 7 September 2006
to do
- check sequences (dye blob re-runs should be in from 9/1 order)
- glycerol 6 new LCs (3 of the 399-1s and 3 of the 320-Cs)
- Y0080 and Y0078 yeast vectors
- miniprep
- digest with XS --->must do the artic freeze step following digest
- run results on a gel
- ligate to J45014:XS (already cut and ready to go in corner of digest tray)
- transform
- pmsCEAB
- digest with ES (has 4 Pst sites!!)
- run results on a gel and clean-up
- ligate into a double resistance backbone:ES (make sure different from w-ever our WGD device is living in)
- transform
- digest with ES (has 4 Pst sites!!)
- pellet new yeast cells (pm) and resuspend in milk -- bake again or bake on saturday
