IGEM:MIT/2006/Notebook/2006-9-8
From OpenWetWare
to do
- start LCs for plate reader?? (SP ?)
- check sequences (dye blob re-runs should be in from 9/1 order)
- glycerol any of the LCs in fridge that look good post this second sequence analysis
- discard of confirmed bad dna and glycerols!
- glycerol 6 new LCs (3 of the 399-1s and 3 of the 320-Cs)
- Y0080 and Y0078 yeast vectors
- miniprep
- digest with XS --->must do the artic freeze step following digest
- run results on a gel
- ligate to J45014:XS (already cut and ready to go in corner of digest tray)
- transform
- pmsCEAB
- digest with ES (has 4 Pst sites!! i said something about Eco in an email, but they are definately Pst!)
- run results on a gel and clean-up
- ligate into a double resistance backbone:ES (make sure different from w-ever our WGD device is living in)
- transform
- digest with ES (has 4 Pst sites!! i said something about Eco in an email, but they are definately Pst!)
- pellet new yeast cells (pm) and resuspend in milk -- bake again or bake on saturday (KB in pm)