IGEM:MIT/2006/Notebook/2007-2-1: Difference between revisions

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10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut)
10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut)


11. Digest Q119B prep to connect to osmYF (precut with ES)
11. Digest Q119B prep to connect to osmYF (precut with ES)- DONE

Revision as of 14:28, 1 February 2007

Plan

1. Smell J45600 hopefuls from last night- DONE (NONE SMELL!!! NO!!!)

2. Miniprep J45180 for further digestion/ligation/transformation- DON"T DO ON ACCOUNT OF PLATE READER RESULTS

3. Heat shock the J45180 overnight digests- DONE

4. Run the overnight digests on a gel to determine which enzyme is/isn't cutting- DONE (XbaI isn't cutting)

5. Graph/analyze plate reader results- DONE (J45998 is not working)

6. Ask Veena about isoamyl acetate GC results

7. Contact Li Li about a good time to run our samples with the internal standard- DONE

8. Extract with n-heptane a negative control for the GC- DONE

9. Ran some samples working towards a calibration curve for methyl salicylate with the GC- DONE

10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut)

11. Digest Q119B prep to connect to osmYF (precut with ES)- DONE

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