IGEM:MIT/2006/Notebook/2007-2-1: Difference between revisions
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5. Graph/analyze plate reader results- DONE (J45998 is not working) | 5. Graph/analyze plate reader results- DONE (J45998 is not working) | ||
6. | 6. Obtain isoamyl acetate results- VEENA WILL DO THIS LATER | ||
7. Contact Li Li about a good time to run our samples with the internal standard- DONE | 7. Contact Li Li about a good time to run our samples with the internal standard- DONE | ||
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8. Extract with n-heptane a negative control for the GC- DONE | 8. Extract with n-heptane a negative control for the GC- DONE | ||
9. Ran | 9. Ran several samples working towards a calibration curve for methyl salicylate with the GC- DONE | ||
10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut) | 10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut) | ||
11. Digest Q119B prep to connect to osmYF (precut with ES)- DONE | 11. Digest Q119B prep to connect to osmYF (precut with ES)- DONE | ||
12. Submit plates to the Registry- DONE | |||
13. Gel extract cut J45250 and J45400- SAVE FOR TOMORROW | |||
14. Make reservations for Li Li's GC tomorrow- DONE | |||
Latest revision as of 19:51, 1 February 2007
Plan
1. Smell J45600 hopefuls from last night- DONE (NONE SMELL!!! NO!!!)
2. Miniprep J45180 for further digestion/ligation/transformation- DON"T DO ON ACCOUNT OF PLATE READER RESULTS
3. Heat shock the J45180 overnight digests- DONE
4. Run the overnight digests on a gel to determine which enzyme is/isn't cutting- DONE (XbaI isn't cutting)
5. Graph/analyze plate reader results- DONE (J45998 is not working)
6. Obtain isoamyl acetate results- VEENA WILL DO THIS LATER
7. Contact Li Li about a good time to run our samples with the internal standard- DONE
8. Extract with n-heptane a negative control for the GC- DONE
9. Ran several samples working towards a calibration curve for methyl salicylate with the GC- DONE
10. Check sequencing to see if J45180 had a bad XbaI site- DONE (YES, IT DID; there is nothing wrong with our enzymes, there was just a mutation which did not allow the enzyme to cut)
11. Digest Q119B prep to connect to osmYF (precut with ES)- DONE
12. Submit plates to the Registry- DONE
13. Gel extract cut J45250 and J45400- SAVE FOR TOMORROW
14. Make reservations for Li Li's GC tomorrow- DONE
