IGEM:MIT/2007/Notebook/2007-6-29: Difference between revisions
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**Research "red team" stuff -- ion exchange | **Research "red team" stuff -- ion exchange | ||
**Brian -- stainless steel, nylon binding? | **Brian -- stainless steel, nylon binding? | ||
**Check on FhuA and pSB3K3 colonies | |||
*Afternoon | *Afternoon | ||
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*Question to think about: metal ion diffusion into the cell or binding at the surface? | *Question to think about: metal ion diffusion into the cell or binding at the surface? | ||
==pSB3K3== | |||
== | *9AM - Good news, all of the colonies we picked last night are cloudy today! :D | ||
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Latest revision as of 06:15, 4 July 2007
Agenda for Friday, June 29
- Morning
- pBAD pickup
- Is OmpA crucial to bacterial cell survival?
- Finalize oligos (restriction sites)
- Research "red team" stuff -- ion exchange
- Brian -- stainless steel, nylon binding?
- Check on FhuA and pSB3K3 colonies
- Afternoon
- Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
- Order above oligo
- Find/finalize vector (pBAD33?)
- Have everything ordered by the end of today!!
- Question to think about: metal ion diffusion into the cell or binding at the surface?
pSB3K3
- 9AM - Good news, all of the colonies we picked last night are cloudy today! :D
