IGEM:MIT/2007/Notebook/2007-6-29

Agenda for Friday, June 29

• Morning
  • pBAD pickup
  • Is OmpA crucial to bacterial cell survival?
  • Finalize oligos (restriction sites)
  • Research "red team" stuff -- ion exchange
  • Brian -- stainless steel, nylon binding?

• Afternoon
  • Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
  • Order above oligo
  • Find/finalize vector (pBAD33?)
  • Have everything ordered by the end of today!!

• Question to think about: metal ion diffusion into the cell or binding at the surface?

Display Proteins

• mRFP Derivatives
  • mRFP1 The original
  • tdTomato

1. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, and Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. DOI:10.1038/nbt1037 |