IGEM:MIT/2007/Notebook/2007-7-19

Agenda for Thursday, 7/19

• Make permanent glycerol stocks of 8 tubes
• AK/AL list
• Colony PCR on yesterday's plated transformation (F2620+B0034+CPX in pSB1AC3)

Colony Count of Yesterday's Plates

TK's DH5a transformed with F2620+B0034+CPX in pSB1AC3

Key:

• (+) contains DH5a transformed with digested and ligated parts
• (-) contains DH5a transformed with only digested and ligated pSB1AC3
• BH the plate is labeled with Bernice's initials
• JH the plate is labeled with Jess's initials

Plate                              #Colonies     Notes
(+) Cm        BH                   ~50           A couple satellite colonies
(+) Cm + Kan  BH                   1             This plate hardened imperfectly
(+) Cm        JH                   ~64
(+) Cm + Kan  JH                   3             On the lip of the plate
(-) Cm                             9             
(-) Cm + Kan                       0

Permanent Glycerol Stocks of DB3.1, F2620, B0034 and E1010

• SK: Made eight cryogenic vials of glycerol stock; all have 1 mL 40% glycerol + 1 mL liquid sample
• All vials in the white sticker labeled "iGEM 2007 DH5alpha" -80 degree freezer box
• DB3.1, 2 vials = original with pSB3K3 (K+)
• F2620, 2 vials = transformed (A+)
• B0034, 2 vials = transformed (A+)
• E1010, 2 vials = transformed (K+)

Liquid Culture of BBa_I13500

• Last night's growth was unsuccessful, so it was redone