IGEM:MIT/2007/Notebook/2007-7-3: Difference between revisions
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Line 6: | Line 6: | ||
*Discuss results of Monday's 2PM meeting | *Discuss results of Monday's 2PM meeting | ||
*Set up meeting with Drew/Tom/grads? | *Set up meeting with Drew/Tom/grads? | ||
==Wet Work== | |||
*All plates look like sludge | |||
**Not sure why | |||
**Could be need to use LB+KAN for recovery step | |||
**Plating with 1000 µl instead of 100 µl | |||
==Make LB+KAN agar plates== | |||
#Heat LB+KAN agar plates | |||
#*Heat LB + Agar 1.2% for 10 minutes at 50% power | |||
#*Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration | |||
#*Pour into labeled plates | |||
==Transform competent bacteria== |
Revision as of 07:51, 3 July 2007
Agenda for July 3
- Call Geneart
- Results of Alex/Diti PCR?
- For metals: RBS and promoter? (clarify!)
- Barry's promoter -- obtainment?
- Discuss results of Monday's 2PM meeting
- Set up meeting with Drew/Tom/grads?
Wet Work
- All plates look like sludge
- Not sure why
- Could be need to use LB+KAN for recovery step
- Plating with 1000 µl instead of 100 µl
Make LB+KAN agar plates
- Heat LB+KAN agar plates
- Heat LB + Agar 1.2% for 10 minutes at 50% power
- Wait until cool to touch and put in 960 µL Kanamycin to make 10 µg/ml concentration
- Pour into labeled plates