IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/02: Difference between revisions
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* 9am - Will make chemically competent DH5a cells. | * 9am - Will make chemically competent DH5a cells. | ||
== <b>Date: 09/30/13</b> <b>People in lab: Levi Palmer</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> Test 11/2 competent cells for competence | |||
<b>Start Time:</b> 5:48 PM | |||
<b>Purpose:</b> To test for competence of cells by transformation | |||
<b>Protocol:</b> LTM ed. 2 | |||
<b>Products:</b> | |||
{| border="1" cellpadding="5" | |||
|- | |||
! Sample Label !! Description !! Source Label !! Quantity | |||
|- | |||
| 11/2/13 G1 control CT || 11/2 competent cells with pUC19, not grown on antibiotic, positive control || 11/2 G1 pUC19 CT || 4 | |||
|- | |||
| 11/2/13 G1 pUC19 CT || 11/2 competent cells with pUC19, grown on Ampicillin || 11/2 G5 pUC19 CT || 4 | |||
|} | |||
<b>Results:</b> Unsure results, lawn growth, retest with negative control | |||
<b>Stop Time:</b> 9:00 PM | |||
Revision as of 16:02, 17 May 2014
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Making Competent CellsPersons participating: Levi Palmer, Emily Puleo, Kelsey Crossen
Date: 09/30/13 People in lab: Levi PalmerTitle: Test 11/2 competent cells for competence Start Time: 5:48 PM Purpose: To test for competence of cells by transformation Protocol: LTM ed. 2 Products:
Results: Unsure results, lawn growth, retest with negative control Stop Time: 9:00 PM
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