IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/11/02

iGEM Project name 1

Main project page Previous entry      Next entry

Making Competent Cells Persons participating: Levi Palmer, Emily Puleo, Kelsey Crossen 9am - Will make chemically competent DH5a cells. Date: 11/2/13 People in lab: Levi Palmer Title: Test 11/2 competent cells for competence Start Time: 5:48 PM Purpose: To test for competence of cells by transformation Protocol: LTM ed. 2 Products: Sample Label Description Source Label Quantity 11/2/13 G1 control CT 11/2 competent cells with pUC19, not grown on antibiotic, positive control 11/2 G1 pUC19 CT 4 11/2/13 G1 pUC19 CT 11/2 competent cells with pUC19, grown on Ampicillin 11/2 G5 pUC19 CT 4 Results: Unsure results, lawn growth, retest with negative control Stop Time: 9:00 PM