IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/03/06: Difference between revisions

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(Autocreate 2014/03/06 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 03/06/14</b> <b>People in lab: Levi Palmer</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Miniprep of hmp from 2/25 TOPO


<b>Start Time:</b>
<b>Start Time:</b> 12:00 PM


<b>Purpose:</b>  
<b>Purpose:</b> To purify the hmp plasmid from the transformed cells to test for correct part by digest


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> LTM ed. 2


<b>Products:</b>  
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
|-
|-
| Entry || Entry || Entry || #
| Poss hmp 3/6 || Purified DNA from possible hmp || Poss. hmp 2/25 || 1
|-
| Entry || Entry || Entry || #
|}
|}


{| border="1" cellpadding="5"
<b>Results:</b> 1: Conc. 45 ng/uL, 280/260: 2, 230/260: 2
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample ||  ||  ||  ||  ||  ||  ||  ||
|}
 
<b>Results:</b>  


<b>Notes:</b>  
<b>Notes:</b>  


<b>Stop Time:</b>  
<b>Stop Time:</b> 4:00PM


<b>Next:</b>
<b>Next:</b> Digest to check for hmp gene by electrophoresis




Revision as of 21:02, 17 May 2014

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Date: 03/06/14 People in lab: Levi Palmer

Title: Miniprep of hmp from 2/25 TOPO

Start Time: 12:00 PM

Purpose: To purify the hmp plasmid from the transformed cells to test for correct part by digest

Protocol: LTM ed. 2

Products:

Sample Label Description Source Label Quantity
Poss hmp 3/6 Purified DNA from possible hmp Poss. hmp 2/25 1

Results: 1: Conc. 45 ng/uL, 280/260: 2, 230/260: 2

Notes:

Stop Time: 4:00PM

Next: Digest to check for hmp gene by electrophoresis



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