IGEM:Peking University/2008/Experiment/PKU 0605 G2 1: Difference between revisions
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Latest revision as of 21:49, 11 July 2008
- Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
Volumn(μL) | |
pUC18 | 5 |
HindIII | 0.2 |
EcoRI | 0.2 |
M Buffer | 2 |
ddH2O | 12.6 |
- Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
Volumn(μL) | |
Gal4 | 15 |
HindIII | 0.2 |
EcoRI | 0.2 |
M Buffer | 5 |
ddH2O | 29 |
- Ligation-pUC18/Gal4
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