IGEM:Peking University/2008/Experiment/PKU 0605 G2 1

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Enzyme cutting-pUC18-HindIII-EcoRI-20 μL


	Volumn(μL)
pUC18	5
HindIII	0.2
EcoRI	0.2
M Buffer	2
ddH₂O	12.6

Enzyme cutting-Gal4-HindIII-EcoRI-50 μL


	Volumn(μL)
Gal4	15
HindIII	0.2
EcoRI	0.2
M Buffer	5
ddH₂O	29

Ligation-pUC18/Gal4

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