IGEM:Peking University/2008/Experiment/PKU 0807014 G1 1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Wenjun Shao (talk | contribs) |
Wenjun Shao (talk | contribs) |
||
Line 43: | Line 43: | ||
[[Image:PKU_080714_lacS-tet-pGREG504cut.tif|center|400px]] | [[Image:PKU_080714_lacS-tet-pGREG504cut.tif|center|400px]] | ||
|} | |} | ||
/*''lanes:lacS|tet|vector|marker1|marker2'' | |||
Revision as of 09:30, 14 July 2008
PKU_080713_G1_1
- Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
- Enzyme cutting-pADH-(lacS/tet)(PCR product)
Volume (μL) | |
pADH-(lacS/tet) | 14 |
SacI | 2 |
XhoI | 2 |
M Buffer | 10 |
ddH2O | 72 |
- Enzyme cutting-pGREG504-1
Volume (μL) | |
pGREG504-1 | 4 |
SacI | 2 |
XhoI | 2 |
M Buffer | 10 |
ddH2O | 82 |
/*lanes:lacS|tet|vector|marker1|marker2
- Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
Volume (μL) | |
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3) | 5 |
SacI | 1 |
XhoI | 1 |
M Buffer | 2 |
ddH2O | 11 |
Volume (μL) | |
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2) | 5 |
SphI | 0.2 |
XhoI | 0.2 |
H Buffer | 2 |
ddH2O | 12.6 |
Volume (μL) | |
pGREG504-pADH-(Control1/Control2/Control3) | 5 |
BamHI | 0.2 |
XhoI | 0.2 |
K Buffer | 2 |
ddH2O | 12.6 |