IGEM:Peking University/2008/Notebook/Group 2/2008/06/05: Difference between revisions

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(Autocreate 2008/06/05 Entry for IGEM:Peking_University/2008/Notebook/Group_2)
 
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==Entry title==
==06-05==
* Insert your content here.
::'''Enzyme cutting-pUC18-HindIII-EcoRI-20 μL'''
 
{|border="1" cellspacing="0"
|+
|-
| ||Volumn(μL)
|-
| pUC18 ||5
|-
| HindIII||0.2
|-
| EcoRI||0.2
|-
| M Buffer||2
|-
|ddH<sub>2</sub>O||12.6
|}
<hr class=divider>
::'''Enzyme cutting-Gal4-HindIII-EcoRI-50 μL'''
{|border="1" cellspacing="0"
|+
|-
| ||Volumn(μL)
|-
| Gal4||15
|-
| HindIII||0.2
|-
| EcoRI||0.2
|-
| M Buffer||5
|-
|ddH<sub>2</sub>O||29
|}
<hr class=divider>
::''' Ligation-pUC18/Gal4'''




Revision as of 12:18, 22 July 2008

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06-05

Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
Volumn(μL)
pUC18 5
HindIII 0.2
EcoRI 0.2
M Buffer 2
ddH2O 12.6
Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
Volumn(μL)
Gal4 15
HindIII 0.2
EcoRI 0.2
M Buffer 5
ddH2O 29
Ligation-pUC18/Gal4



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