IGEM:PennState/2007

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<p>The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT.  The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006  
<p>The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT.  The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006  
jamboree consisted of 37 teams from 12 countries whom presented their findings.</p>
jamboree consisted of 37 teams from 12 countries whom presented their findings.</p>
-
<p>The PSU 2007 iGEM team is currently developing projects radiation detection and ethanol production. More information can be found in the Project Section.</p>  
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<p>
 +
Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie.  Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests.  Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose.  The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.</p>  
<p>If you are interested in joining the team [[IGEM:PennState/Newmember|click here]].</p></font>
<p>If you are interested in joining the team [[IGEM:PennState/Newmember|click here]].</p></font>
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*[[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[user:luw134|<font color="#CCFFFF">Lucien Weiss</font>]]
*[[user:luw134|<font color="#CCFFFF">Lucien Weiss</font>]]
 +
*[[user:samhita_banavar|<font color="#CCFFFF">Samhita Banavar</font>]]
<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2>
<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2>
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*[[IGEM:PennState/2006/user:Jbig|<font color="#CCFFFF">John Bigus</font>]]  
*[[IGEM:PennState/2006/user:Jbig|<font color="#CCFFFF">John Bigus</font>]]  
*[[IGEM:PennState/2006/user:Jdun|<font color="#CCFFFF">Jen Dunning</font>]]
*[[IGEM:PennState/2006/user:Jdun|<font color="#CCFFFF">Jen Dunning</font>]]
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*[[IGEM:PennState/2007/user:GKho|<font color="#CCFFFF">George Khoury</font>]]
<h2><font color="#FFFFFF">Graduate Students</font></h2>
<h2><font color="#FFFFFF">Graduate Students</font></h2>
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*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Megan Marshall</font>]]
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*[[IGEM:PennState/2006/user:Megan_Marshall|<font color="#CCFFFF">Megan Marshall</font>]]
*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Deepti Tanjore</font>]]
*[[IGEM:PennState/2006/user:Dtanjore|<font color="#CCFFFF">Deepti Tanjore</font>]]
*[[IGEM:PennState/2006/user:SWal|<font color="#CCFFFF">Steve Walker</font>]]
*[[IGEM:PennState/2006/user:SWal|<font color="#CCFFFF">Steve Walker</font>]]
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<h2><font color="#FFFFFF">Faculty</font></h2>
<h2><font color="#FFFFFF">Faculty</font></h2>
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*[[IGEM:PennState/2006/user:MTien|<font color="#CCFFFF">Ming Tien</font>]]   
*[[IGEM:PennState/2006/user:MTien|<font color="#CCFFFF">Ming Tien</font>]]   
*[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]]
*[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]]
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<h2><font color="#FFFFFF">Photos</font></h2>
<h2><font color="#FFFFFF">Photos</font></h2>
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*[[IGEM:PennState/2007/Results|<font color="#CCFFFF">Results</font>]]
*[[IGEM:PennState/2007/Results|<font color="#CCFFFF">Results</font>]]
*[[IGEM:PennState/2007/Graphics|<font color="#CCFFFF">Graphics</font>]]
*[[IGEM:PennState/2007/Graphics|<font color="#CCFFFF">Graphics</font>]]
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<h2><font color="#FFFFFF">Lab Notebooks</font></h2>
<h2><font color="#FFFFFF">Lab Notebooks</font></h2>
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*[[IGEM:PennState/Labbook/CommonBook|<font color="#CCFFFF">General Lab Notebook</font>]]
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*[[IGEM:PennState/Labbook/ChrisBuckno|<font color="#CCFFFF">Chris Buckno</font>]]
*[[IGEM:PennState/Labbook/JoelQ.Grim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/Labbook/JoelQ.Grim|<font color="#CCFFFF">Joel Q. Grim</font>]]
*[[IGEM:PennState/Labbook/NoahJohnson|<font color="#CCFFFF">Noah Johnson</font>]]
*[[IGEM:PennState/Labbook/NoahJohnson|<font color="#CCFFFF">Noah Johnson</font>]]
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*[[IGEM:PennState/Labbook/GarrettTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[IGEM:PennState/Labbook/GarrettTobin|<font color="#CCFFFF">Garrett Tobin</font>]]
*[[IGEM:PennState/Labbook/LucienWeiss|<font color="#CCFFFF">Lucien Weiss</font>]]
*[[IGEM:PennState/Labbook/LucienWeiss|<font color="#CCFFFF">Lucien Weiss</font>]]
 +
*[[IGEM:PennState/Labbook/SamhitaBanavar|<font color="#CCFFFF">Samhita Banavar</font>]]
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<h2><font color="#FFFFFF">Fun Stuff</font></h2>
<h2><font color="#FFFFFF">Fun Stuff</font></h2>
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<html><a href="http://www2.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/IGEM:PennState" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/IGEM:PennState" /></a>
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<html><a href="http://www2.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/IGEM:PennState&type=small&category=free&clusters=no&map=world" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/IGEM:PennState" /></a>
<br>
<br>
<br>
<br>
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<h2>Protocols</h2>
<h2>Protocols</h2>
 +
*[[IGEM:PennState/2006/Growthmedia|<font color="#000000">Growth Media</font>]]
*[[IGEM:PennState/2006/Cloning|<font color="#000000">Cloning</font>]]  
*[[IGEM:PennState/2006/Cloning|<font color="#000000">Cloning</font>]]  
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000000">Preparing chemically competent cells</font>]]   
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000000">Preparing chemically competent cells</font>]]   
**[[IGEM:PennState/2006/GrowingParts|<font color="#000000">Growing Parts</font>]]  
**[[IGEM:PennState/2006/GrowingParts|<font color="#000000">Growing Parts</font>]]  
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**[[IGEM:PennState/2007/Dnaprep|<font color="#000000">Small Scale Preparation of Genomic DNA</font>]]  
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**[[IGEM:PennState/2007/Dnaprep|<font color="#000000">Telt Method DNA Prep</font>]]  
**[[IGEM:PennState/2006/Restriction|<font color="#000000">Restriction</font>]]  
**[[IGEM:PennState/2006/Restriction|<font color="#000000">Restriction</font>]]  
**[[IGEM:PennState/2006/Ligation|<font color="#000000">Ligation</font>]]  
**[[IGEM:PennState/2006/Ligation|<font color="#000000">Ligation</font>]]  
**[[IGEM:PennState/2006/Transformation|<font color="#000000">Transformation</font>]]
**[[IGEM:PennState/2006/Transformation|<font color="#000000">Transformation</font>]]
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*[[IGEM:PennState/2006/Lambda Techniques|<font color="#000000">Lambda Techniques</font>]]
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*[[IGEM:PennState/2006/dnagels|<font color="#000000">Agarose Gel</font>]]
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**[[IGEM:PennState/2006/sybrgreen|<font color="#000000">Working with Sybr Green I</font>]]
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*[http://openwetware.org/wiki/Silver:_PCR <font color="#000000">PCR</font>]
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**[[IGEM:PennState/2007/FreezeandSqueeze|<font color="#000000">PCR Purification Freeze and Squeeze</font>]]
*[[IGEM:PennState/2006/Plate-Reading|<font color="#000000">Plate-reading</font>]]
*[[IGEM:PennState/2006/Plate-Reading|<font color="#000000">Plate-reading</font>]]
*[[IGEM:PennState/2006/StrainConstruction|<font color="#000000">Strain Construction</font>]]   
*[[IGEM:PennState/2006/StrainConstruction|<font color="#000000">Strain Construction</font>]]   
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*[[IGEM:PennState/2006/PSUinventory|<font color="#000000">Inventory</font>]]
 
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*[[IGEM:PennState/2006/dnagels|<font color="#000000">DNA Gel</font>]]
 
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*[[IGEM:PennState/2006/Growthmedia|<font color="#000000">Growth Media</font>]]
 
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*[[IGEM:PennState/2006/LBPlates|<font color="#000000">LB Plates</font>]]
 
*[[IGEM:PennState/2006/Eikenplates|<font color="#000000">Eiken Agar Plates</font>]]
*[[IGEM:PennState/2006/Eikenplates|<font color="#000000">Eiken Agar Plates</font>]]
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*[http://openwetware.org/wiki/Silver:_PCR <font color="#000000">PCR</font>]
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*[[IGEM:PennState/2006/PSUinventory|<font color="#000000">Inventory</font>]]
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*[http://www.bios.niu.edu/core/freeze_squeeze.htm <font color="#000000">PCR Purification Freeze and Squeeze</font>]
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*[http://169.237.98.99/protocols/D16.html <font color="#000000'>TELT plasmid prep</font>]
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<h2>Progress</h2>
<h2>Progress</h2>
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*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000000">Submitted Registry Parts</font>]]
*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000000">Submitted Registry Parts</font>]]
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000000">Microcentrifuge Tube Lists</font>]]
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000000">Microcentrifuge Tube Lists</font>]]
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*[[IGEM:PennState/2006/Progress_microchannels|<font color="#000000">Microchannels results</font>]]
 
*[[IGEM:PennState/2006/Progress_motility|<font color="#000000">Motility results</font>]]
*[[IGEM:PennState/2006/Progress_motility|<font color="#000000">Motility results</font>]]
 +
*[[IGEM:PennState/2007/Storage|<font color="#000000">Glycerol Stock Inventory</font>]]
<h2>Background</h2>
<h2>Background</h2>
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<h2>Links</h2>
<h2>Links</h2>
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*[[iGEM|iGEM on OpenWetWare]]
 
*[http://parts.mit.edu/registry Parts registry]
*[http://parts.mit.edu/registry Parts registry]
*[http://parts.mit.edu/igem iGEM wiki]
*[http://parts.mit.edu/igem iGEM wiki]
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*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]
*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]
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*[http://lscfac.biotec.psu.edu/oligo/order.lasso Sequencing Facility]
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*[http://tanager.biotec.psu.edu/ Oligo orders]
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 Lambda Phage Genome]
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 Lambda Phage Genome]
 +
*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html Ethanol Precipitation]
 +
*[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi]
 +
*[http://www.sciencegateway.org/tools/bacteria.htm OD Calculator]
|}
|}

Current revision

Image:Psuigemlogo2007.jpg

Image:PSUiGEM2007OldMain.jpg

The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of a registry of parts. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The 2006 jamboree consisted of 37 teams from 12 countries whom presented their findings.

Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.

If you are interested in joining the team click here.

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