IGEM:PennState/2007/News/MeetingNotes
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Monday, May 21-Group Meeting in 244 Ag Engineering 3PM More information about the meetings below. If times do not work, please let me know and we can reschedule them to a better time. Also, if anyone has a copy of the Genetic Switch and would like to lend it out to one of our 3 new members please let me know, and I will get it to one of them as quickly as possible.
At the Student Meeting
Friday, May 18-Student Meeting at 408 Althouse 2PM
We went over:
- using the spectrophotometer
- locations of lab supplies
- general lab strategies
- planned brainstorming session
Monday, May 21-Group Meeting in 244 Ag Engineering 3PM
Summer Schedule
Monday, May 30-Group Meeting in 244 Ag Engineering 4PM
Todays Topic: Project Propoasals
- Friday is decision deadline
Suggestions: Focus on areas under advisors expertise, focus on iGEM objectives (Energy, Fuel) iGEM objectives for 2007
- Health and Medicine
- Energy and Environment
- Parts and Devices
Project Proposals
- Radiation Biosensor
- Heat Biosensor
- Exothermic Enzymes
- Biofilms
- Tom Richards: Applications to Synthetic Biology
- Renewable Energy Biomass:
- Problem is Multiple Sugar Types
- Diauxie
- Remove Diauxie by modifying bacteria to remove both sugars at the same time.
- Problem is Multiple Sugar Types
Saturday, June 2-Undergraduate Online Meeting 4PM
- What is the composition of the biomass (sugars x%)?
- Is there a biomass standard
- How do we knock the xylose metabolism parts out of the chromosome
- Put xyl A, B, E under control of xylR
- Do we we want the xylose metabolism to be under tight repression?
- Should we be looking at multiple sugar metabolizing pathways?
Plan:
- Noah/Garrett-look up biomass papers come up with questions
- Luc-come up with weekly plan
Sunday, June 3-Undergraduate Meeting HUB 12PM
Monday, June 4-Group Meeting in 244 Ag Engineering 4PM
- Biobrick-ing
- Just need to order primers, will have biobricks on them
- Have to add a silent mutation to use restriction enzymes on so that you dont accidentally cut the gene
- Should probably make 2 separate biobricks:
- 1) All the way from xylF to just before xylA starts
- 2) Smaller one: remove crp binding site, possibly put in a spacer to preserve looping mechanism
- This might be a pain to PCR: might want to order it if we go that route
- Diauxie Project
- Use xylE instead of xylF,G,H (smaller and easier to work with)
- Ptac = mixture of lac and trp
- If xylR acts like araC we need to keep an upstream area to allow it to loop correctly (~200bp)
- Fis? - Noone knew how it worked or if its necessary... found short description from 2006 iGEM, also paper describing Fis listed on ANGEL under Diauxie
- NAD+ Chromosomal deletion- good idea, research further
- Biomass- Glucose and Xylose are ~80-90%
- Don't need to worry about repressing crp because majority of dimers will be crp* so the overwhelming concentration will factor out crp
- Dosimeter Project
- Currently a UV lysing component is used on plasmids
- Does it use recA? ...should look into it
- Part BBa R0011 - Lambda switch
- Could consider biobrick-ing recA
- Currently a UV lysing component is used on plasmids
- Next Steps:
- Biobrick xylR first separately, biobrick other components mentioned
- Look into cloning software (CloneManager), If not possible to get on Richard Lab computers we can use Dr. Cirino's computers in his lab sometime
- Order DNA synthesis soon
- Research feasibility of dosimeter
- Continue researching diauxie project.. Dr. C will send more papers