IGEM:PennState/2008: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
[[Image: | [[Image:Psuigemlogo2008.jpg]] | ||
[[Image:PSUiGEM2007OldMain.jpg]] | [[Image:PSUiGEM2007OldMain.jpg]] | ||
| | <h2 style="color: #fff">What iGEM is All About</h2> | ||
<p style="color: #fff">The International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of an open registry of parts, or biobricks. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The [[http://parts.mit.edu/igem07/index.php/Main_Page| 2007 Jamboree]] consisted of 54 teams from 19 countries who presented their findings.</p> | |||
<h2 style="color: #fff">Penn State's 2007 iGEM project: Diauxie Elimination</h2> | |||
<p style="color: #fff">Penn State won a Gold medal in the 2007 iGEM Jamboree! We're proud of our work during the year and our registry submissions.</p> | |||
<h3 style="color: #fff">Abstract:</h3> | |||
<p style="color: #fff">Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions.</p> | |||
<h2 style="color: #fff">Want to Join?</h2> | |||
<p style="color: #fff">If you are interested in joining the team [[IGEM:PennState/Newmember|click here]].</p></font> | |||
<font color="#FFFFFF" size="6"> | <font color="#FFFFFF" size="6"> | ||
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<h2><font color="#FFFFFF">Fulltime Undergraduates</font></h2> | <h2><font color="#FFFFFF">Fulltime Undergraduates</font></h2> | ||
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|- | |||
|class="teamm"| [[user:GTobin|<font color="#CCFFFF">Garrett Tobin</font>]] | |||
|[[IGEM:PennState/Labbook/GarrettTobin08|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|- | |||
|class="teamm"|[[user:Trevor Wiley|<font color="#CCFFFF">Trevor Wiley</font>]] | |||
|[[IGEM:PennState/Labbook/Trevor Wiley|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|- | |||
|class="teamm"|[[user:Nimrah Ahmed|<font color="#CCFFFF">Nimrah Ahmed</font>]] | |||
|[[IGEM:PennState/Labbook/Nimrah Ahmed|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|- | |||
|class="teamm"|[[user:yourusernamehere|<font color="#CCFFFF">Daniel Seong</font>]] | |||
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|- | |||
|class="teamm"|[[user:Shariful Alam|<font color="#CCFFFF">Shariful Alam</font>]] | |||
|[[IGEM:PennState/Labbook/Shariful Alam|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|- | |||
|class="teamm"|[[user:yourusernamehere|<font color="#CCFFFF">Berooke Alemayehu</font>]] | |||
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]] | |||
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<h2><font color="#FFFFFF">High School Student</font></h2> | |||
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|class="teamm"| [[user:samhita_banavar|<font color="#CCFFFF">Samhita Banavar</font>]] | |||
|[[IGEM:PennState/Labbook/GarrettTobin08|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|} | |||
<h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2> | <h2><font color="#FFFFFF">Affiliated Undergraduates</font></h2> | ||
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|- | |||
|[[IGEM:PennState/2007/user:GKho|<font color="#CCFFFF">George Khoury</font>]] || | |||
|- | |||
|class="teamm"|[[user:Galen Lynch|<font color="#CCFFFF">Galen Lynch</font>]] | |||
|[[IGEM:PennState/Labbook/yourname|<font color="#CCFFFF">Lab Notebook</font>]] | |||
|} | |||
<h2><font color="#FFFFFF"> | <h2><font color="#FFFFFF">Advisory Assistance</font></h2> | ||
*[[IGEM:PennState/2006/user:Megan_Marshall|<font color="#CCFFFF">Megan Marshall</font>]] | *[[IGEM:PennState/2006/user:Megan_Marshall|<font color="#CCFFFF">Megan Marshall</font>]] | ||
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*[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]] | *[[IGEM:PennState/2006/user:PW|<font color="#CCFFFF">Paul Weiss</font>]] | ||
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<html><font face="Tahoma" color="#FFFFFF" size="6"><blink>></blink>Project</font> | |||
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*[http://2008.igem.org/Team:PennState <font color="#CCFFFF">2008 Penn State iGEM Project Site</font>] | |||
*[http://2008.igem.org/Team:PennState/Notebook <font color="#CCFFFF">2008 Lab Notebook</font>] | |||
<h2>In The News</h2> | <h2>In The News</h2> | ||
*[[IGEM:PennState/Publications/Synthetic Sports: A Bacterial Relay Race|<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]] | *[[IGEM:PennState/Publications/Synthetic Sports: A Bacterial Relay Race|<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]] | ||
*[[IGEM:PennState/Publications/Posters | *[[IGEM:PennState/Publications/Posters <font color="#000">PSU iGEM Posters</font>]] | ||
*[https://www.engr.psu.edu/NewsEvents/EPS/v22n2_2006spring/igem.htm<font color="# | *[https://www.engr.psu.edu/NewsEvents/EPS/v22n2_2006spring/igem.htm<font color="#000">Ready. Set. Grow!?</font>] | ||
*[http://www.ainewsletter.com/newsletters/aix_0512.htm#biobricks_conjugate <font color="# | *[http://www.ainewsletter.com/newsletters/aix_0512.htm#biobricks_conjugate <font color="#000">AI Newsletter</font>] | ||
*[http://publications.nigms.nih.gov/findings/mar07/findings_mar07.pdf <font color="# | *[http://publications.nigms.nih.gov/findings/mar07/findings_mar07.pdf <font color="#000">Government Report</font>] | ||
*[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="# | *[http://www.mrsec.psu.edu/highlights/publications/publications2006.pdf <font color="#000">MRSEC Publications</font>] | ||
<h2>Tasks</h2> | <h2>Tasks</h2> | ||
*[[IGEM:PennState/News/WebsiteTasks|<font color="#000">Website Tasks</font>]] | |||
*[[IGEM:PennState/2007/News/MeetingNotes|<font color="#000">Group Meeting Minutes</font>]] | |||
*[[ | <h2>Team Utilities</h2> | ||
*[ | *[http://tools.neb.com/NEBcutter2/index.php <font color="#000">NEB Cutter</font>] | ||
*[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp <font color="#000">Double Digest Finder</font>] | |||
*[http://tanager.biotec.psu.edu/|<font color="#000">Oligo orders</font>] | |||
*[http://www.geneart.com/|<font color="#000">Plasmid Orders (Geneart)</font>] | |||
*[http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix|<font color="#000">BioBrick Prefix & Suffix</font>] | |||
*[http://www.sciencegateway.org/tools/bacteria.htm <font color="#000">OD Calculator</font>] | |||
<h2>Protocols</h2> | <h2>Protocols</h2> | ||
*[[IGEM:PennState/2006/Growthmedia|<font color="# | *[[IGEM:PennState/2006/Growthmedia|<font color="#000">Growth Media</font>]] | ||
*[[IGEM:PennState/2006/Cloning|<font color="# | *[[IGEM:PennState/2006/Cloning|<font color="#000">Cloning</font>]] | ||
**[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="# | **[[IGEM:PennState/2006/GrowingParts|<font color="#000">Growing Parts (Innoculation)</font>]] | ||
**[[IGEM:PennState/ | **[[IGEM:PennState/2006/PreparingChemicallyCompetentCells|<font color="#000">Preparing chemically competent cells</font>]] | ||
**[[IGEM:PennState/2007/Dnaprep|<font color="# | **[[IGEM:PennState/2008/PreparingElectrocompetentCells|<font color="#000">Preparing Electrocompetent cells</font>]] | ||
**[[IGEM:PennState/2006/Restriction|<font color="# | **[[IGEM:PennState/2007/Dnaprep|<font color="#000">Telt Method DNA Prep</font>]] | ||
**[[IGEM:PennState/2006/Ligation|<font color="# | **[[IGEM:PennState/2006/Restriction|<font color="#000">Restriction</font>]] | ||
**[[IGEM:PennState/2006/Transformation|<font color="# | **[[IGEM:PennState/2006/Ligation|<font color="#000">Ligation</font>]] | ||
*[[IGEM:PennState/2006/ | **[[IGEM:PennState/2006/Transformation|<font color="#000">Transformation</font>]] | ||
*[[IGEM:PennState/ | *[[IGEM:PennState/2006/dnagels|<font color="#000">Agarose Gel</font>]] | ||
**[[IGEM:PennState/2006/sybrgreen|<font color="# | **[[IGEM:PennState/2008/TAEBuffer|<font color="#000">TAE Buffer</font>]] | ||
*[http://openwetware.org/wiki/Silver:_PCR <font color="# | **[[IGEM:PennState/2006/sybrgreen|<font color="#000">Working with Sybr Green I</font>]] | ||
**[[IGEM:PennState/2007/FreezeandSqueeze|<font color="# | *[http://openwetware.org/wiki/Silver:_PCR <font color="#000">PCR</font>] | ||
*[[IGEM:PennState/2006/Plate-Reading|<font color="# | **[[IGEM:PennState/2007/FreezeandSqueeze|<font color="#000">PCR Purification Freeze and Squeeze</font>]] | ||
*[[IGEM:PennState/2006/StrainConstruction|<font color="# | *[[IGEM:PennState/2006/Plate-Reading|<font color="#000">Plate-reading</font>]] | ||
*[[IGEM:PennState/2006/Eikenplates|<font color="# | *[[IGEM:PennState/2006/StrainConstruction|<font color="#000">Strain Construction</font>]] | ||
*[[IGEM:PennState/2006/PSUinventory|<font color="# | *[[IGEM:PennState/2006/Eikenplates|<font color="#000">Eiken Agar Plates</font>]] | ||
*[[IGEM:PennState/2006/PSUinventory|<font color="#000">Inventory</font>]] | |||
<h2>Progress</h2> | <h2>Progress</h2> | ||
*[[IGEM:PennState/2007/Progress_Project_Development|<font color="# | *[[IGEM:PennState/2007/Progress_Project_Development|<font color="#000">Project Development Overview</font>]] | ||
*[[IGEM:PennState/2007/Progress_Cloning|<font color="# | *[[IGEM:PennState/2007/Progress_Cloning|<font color="#000">Cloning</font>]] | ||
*[[IGEM:PennState/2007/Progress_Registry Parts|<font color="# | *[[IGEM:PennState/2007/Progress_Registry Parts|<font color="#000">Submitted Registry Parts</font>]] | ||
*[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="# | *[[IGEM:PennState/2007/Microcentrifuge_tube_lists|<font color="#000">Microcentrifuge Tube Lists</font>]] | ||
*[[IGEM:PennState/2006/Progress_motility|<font color="# | *[[IGEM:PennState/2006/Progress_motility|<font color="#000">Motility results</font>]] | ||
*[[IGEM:PennState/2007/Storage|<font color="# | *[[IGEM:PennState/2007/Storage|<font color="#000">Glycerol Stock Inventory</font>]] | ||
<h2>Background</h2> | <h2>Background</h2> | ||
*[[IGEM:PennState/history|<font color="# | *[[IGEM:PennState/history|<font color="#000">History of Penn State iGEM</font>]] | ||
*[[iGEM:PennState/2006 | <font color="#000">Penn State iGEM 2006 on OpenWetWare</font>]] | |||
*[http://parts.mit.edu/wiki/index.php/Penn_State_2005 Penn State <font color="#000"> iGEM 2005</font>] | |||
*[http://parts.mit.edu/wiki/index.php/Penn_State_University_2006 <font color="#000">Penn State iGEM 2006</font>] | |||
<h2>Links</h2> | <h2>Links</h2> | ||
*[http://parts.mit.edu/registry Parts registry] | *[http://parts.mit.edu/registry <font color="#000">Parts registry</font>] | ||
*[http://parts.mit.edu/igem iGEM wiki | *[http://parts.mit.edu/igem iGEM <font color="#000">wiki</font>] | ||
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 | <font color="#000">Lambda Phage Genome</font>] | |||
*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html <font color="#000"> Ethanol Precipitation</font>] | |||
*[http://www.ncbi.nlm.nih.gov/sites/entrez?db=genome&cmd=Retrieve&dopt=Overview&list_uids=10119&window=7964&begin=10568 Lambda Phage Genome] | |||
*[http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html Ethanol Precipitation] | |||
*[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi] | *[http://0-www.ncbi.nlm.nih.gov.ilsprod.lib.neu.edu/blast/bl2seq/wblast2.cgi] | ||
|} | |} |
Latest revision as of 19:53, 14 July 2008
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What iGEM is All AboutThe International Genetically Engineered Machines (iGEM) Competition is an annual undergraduate research competition hosted by MIT. The project aim is to develop Synthetic Biology through the creation of an open registry of parts, or biobricks. Each part in the registry is an analyzed strain of DNA with several specific restriction sites at each end of the fragment. These strains can be anything from promoters to genes, allowing easy assembly and reassembly of these parts into genetic circuits. The [2007 Jamboree] consisted of 54 teams from 19 countries who presented their findings. Penn State's 2007 iGEM project: Diauxie EliminationPenn State won a Gold medal in the 2007 iGEM Jamboree! We're proud of our work during the year and our registry submissions. Abstract:Increasing energy demands have brought about the need for a renewable, efficient energy source. Using microorganisms to convert biomass to fuel offers a promising alternative to traditional energy sources, but still faces developmental challenges. Microbes such as Escherichia coli have evolved to preferentially metabolize sugars in a process knows as diauxie. Engineering bacteria to eliminate diauxie with the common lignocellulose sugar xylose would allow faster digestion of ordinary plant biomass while simultaneously reducing the costly sugar residues of wild type bacterial digests. Such modified strains of E. coli need to reduce or eliminate glucose’s repression of catabolization proteins necessary to utilize the energy stored in xylose. The effect of such augmentation would be readily assayed with fluorescent proteins placed downstream of xylose regulatory regions. Want to Join?If you are interested in joining the team click here.
Fulltime Undergraduates
High School Student
Affiliated Undergraduates
Advisory AssistanceFaculty
Fun Stuff
<!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=2623386; var sc_invisible=0; var sc_partition=25; var sc_security="dc27ec2c"; </script> <a href="http://my.statcounter.com/project/standard/stats.php?project_id=2623386&guest=1" id="clustrMapsLink"><img src="http://c26.statcounter.com/counter.php?sc_project=2623386&java=0&security=dc27ec2c&invisible=0" alt="blog counter" border="0"" /></a> <br> <br> <SCRIPT LANGUAGE="JAVASCRIPT"><!-- hide this script tag's contents from old browsers today = new Date(); BigDay = new Date("November 3, 2007") msPerDay = 24 * 60 * 60 * 1000 ; timeLeft = (BigDay.getTime() - today.getTime()); e_daysLeft = timeLeft / msPerDay; daysLeft = Math.floor(e_daysLeft); e_hrsLeft = (e_daysLeft - daysLeft)*24; hrsLeft = Math.floor(e_hrsLeft); minsLeft = Math.floor((e_hrsLeft - hrsLeft)*60); document.write(<font color=red>"There are only:<BR> <H4>" + daysLeft + " days " + hrsLeft +" hours and " + minsLeft + " minutes left </H4> Until the 2007 iGEM Jamboree! (Nov. 3rd, 2007)<P>"</font>); // -- done hiding from old browsers --></SCRIPT> </html>
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<html><font face="Tahoma" color="#FFFFFF" size="6"><blink>></blink>Project</font> </html> In The News
Tasks
Team Utilities
ProtocolsProgress
Background
Links
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