IGEM:PennState/Labbook/NoahJohnson/2007-8-20: Difference between revisions
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(New page: <h1>August 20, 2007</h1> PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase) 10-) |
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PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase) | PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase) | ||
10- | Restriction digest of crp* and P1010 in psB1A2 with EcoR1 and SpeI | ||
Ran gel of restrictions: | |||
*All 4 crp* digests worked | |||
*none of the psB1A2 digests seemed to work | |||
10-5:30 | |||
Revision as of 14:25, 20 August 2007
August 20, 2007
PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase)
Restriction digest of crp* and P1010 in psB1A2 with EcoR1 and SpeI
Ran gel of restrictions:
- All 4 crp* digests worked
- none of the psB1A2 digests seemed to work
10-5:30
