IGEM:Stanford/2009/Notebook/Homeostasis/2009/08/04: Difference between revisions
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'''Goals''' | '''Goals''' | ||
* | *Ligate, transform, colony PCR... | ||
'''Project/Accomplishments''' | '''Project/Accomplishments''' | ||
*'''Wet-Lab''' | *'''Wet-Lab''' | ||
**[ | **Looks like success for some first rounds of cloning!!! | ||
***Now we have to do colony PCR to check.... | |||
***Ligation for 1b | |||
**Tonight | |||
***restreaking | |||
***Liquid culture for stuff we are running low on (SoxR, BLH, etc) | |||
***Ran gel from colony PCR | |||
****Re-run in the morning (still getting used to new equipment and it looked funny) | |||
{| | |||
| [[Image:1a.JPG |thumb|left |Results from 1a cloning ]] | |||
| [[Image:1c.JPG |thumb|center |Results from 1c cloning ]] | |||
| [[Image:1d.JPG |thumb|right |Results from 1d cloning ]] | |||
|} | |||
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==Events== | ==Events== | ||
* | *'''Graduate Student Board Meeting at 9am''' | ||
**Feedback | |||
***Better Intro | |||
***Change the first slide, it is scary. We could put into in other parts of the powerpoint | |||
***Too much text on the second slide | |||
***Visual of differentiation of T-cells | |||
***The Movie | |||
**** exaggerate T-cell populations | |||
**** possibly show a graph | |||
****Take out what you are not using... Is it necessary? | |||
****More contrast between before and after the implantation of the device | |||
****Take out the green dots | |||
***Think more about the purpose of our projest | |||
****How is it different from pre-existing medicine | |||
***Explain the location and environment/ symptoms of IBD | |||
***Naming= "NO sensor" "Trp sensor" explain RA- be VERY clear and simple | |||
***How is the device used to treat the disease? Why E. coli? What are the results? | |||
****'''Why is this a good solution?''' - greater context of what you are doing and '''AWESOMENESS!''' | |||
****Sell it! | |||
***The device level diagram slide | |||
****Divide into two different slides | |||
****Reduce the amount of details | |||
**Things to think about | |||
***How many background do we need to give? Background vs. Specifics in Final Presentation | |||
***How many people does this disease effect? | |||
***Talk more about the consequences of implanting the device | |||
***Keep all information simple, imagine your audience as really really lazy people | |||
***Impress your audience vs. communication | |||
***vBOL--talk more about what it is and what it could be used for | |||
**Slideshow general | |||
***3 concepts per slide | |||
***Show the results, not the process | |||
***'''Inroduce vBOL symbols from the start and then use them throughout the presentation''' | |||
**Problems that we have been having | |||
***A biobrick standard that doesn't use Pst1 because the presence of restriction sites in gene sequence (Trp operon) that we can't mutate | |||
***Assign grad students for assay design | |||
***Trp operon--Meeting with Yanofsky | |||
==Comments== | ==Comments== | ||
*[leave a comment!] | *[leave a comment!] | ||
http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning | |||
==Weekly Goals== | ==Weekly Goals== |
Revision as of 10:01, 5 August 2009
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http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning Weekly Goals
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