IGEM:Stanford/2009/Notebook/Homeostasis/2009/08/04: Difference between revisions
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**Looks like success for some first rounds of cloning!!! | **Looks like success for some first rounds of cloning!!! | ||
***Now we have to do colony PCR to check.... | ***Now we have to do colony PCR to check.... | ||
*** | ***Ligation for 1b | ||
**Tonight | |||
***restreaking | |||
***Liquid culture for stuff we are running low on (SoxR, BLH, etc) | |||
***Ran gel from colony PCR | |||
****Re-run in the morning (still getting used to new equipment and it looked funny) | |||
{| | {| | ||
| [[Image:1a.JPG |thumb|left |Results from 1a cloning ]] | | [[Image:1a.JPG |thumb|left |Results from 1a cloning ]] | ||
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==Events== | ==Events== | ||
*Graduate Student Board Meeting at 9am | *'''Graduate Student Board Meeting at 9am''' | ||
**Feedback | **Feedback | ||
***Better Intro | |||
***Change the first slide, it is scary. We could put into in other parts of the powerpoint | ***Change the first slide, it is scary. We could put into in other parts of the powerpoint | ||
***Too much text on the second slide | ***Too much text on the second slide | ||
***Visual of differentiation of T-cells | |||
***The Movie | ***The Movie | ||
****Is it necessary? | **** exaggerate T-cell populations | ||
**** possibly show a graph | |||
****Take out what you are not using... Is it necessary? | |||
****More contrast between before and after the implantation of the device | ****More contrast between before and after the implantation of the device | ||
****Take out the green dots | ****Take out the green dots | ||
***Think more about the purpose of our projest | ***Think more about the purpose of our projest | ||
****How is it different from pre-existing medicine | ****How is it different from pre-existing medicine | ||
****What | ***Explain the location and environment/ symptoms of IBD | ||
***Naming= "NO sensor" "Trp sensor" explain RA- be VERY clear and simple | |||
***How is the device used to treat the disease? Why E. coli? What are the results? | |||
****'''Why is this a good solution?''' - greater context of what you are doing and '''AWESOMENESS!''' | |||
****Sell it! | ****Sell it! | ||
***The device level diagram slide | |||
****Divide into two different slides | |||
****Reduce the amount of details | |||
**Things to think about | **Things to think about | ||
***How many background do we need to give? | ***How many background do we need to give? Background vs. Specifics in Final Presentation | ||
***How many people does this disease effect? | ***How many people does this disease effect? | ||
***Talk more about the consequences of implanting the device | ***Talk more about the consequences of implanting the device | ||
***Keep all information simple, imagine your audience as really really lazy people | ***Keep all information simple, imagine your audience as really really lazy people | ||
***Impress your audience vs. communication | |||
***vBOL--talk more about what it is and what it could be used for | |||
**Slideshow general | |||
***3 concepts per slide | |||
***Show the results, not the process | |||
***'''Inroduce vBOL symbols from the start and then use them throughout the presentation''' | |||
**Problems that we have been having | |||
***A biobrick standard that doesn't use Pst1 because the presence of restriction sites in gene sequence (Trp operon) that we can't mutate | |||
***Assign grad students for assay design | |||
***Trp operon--Meeting with Yanofsky | |||
==Comments== | ==Comments== | ||
*[leave a comment!] | *[leave a comment!] | ||
http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning | |||
==Weekly Goals== | ==Weekly Goals== |
Revision as of 10:01, 5 August 2009
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http://news.yahoo.com/s/ap/20090804/ap_on_bi_ge/us_arthritis_drugs_fda_warning Weekly Goals
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