IGEM:The Citadel/Notebook/Notes Brian/05-30-10: Difference between revisions

Revision as of 14:41, 30 May 2010

This is a continuation of our work from 05-29-10.

- The 250mL tubes were autoclaved with caps off, so to ensure sterility we've stored them on ethanol-soaked paper.

Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37°C with rotation.
Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update! For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox.

@@ Line 8: / Line 8: @@
 #Autoclaved all flasks and tubes.
-#Prechilled all tubes and pipets at 4&deg;C or -80&deg;C as appropriate.
+** The 250mL tubes were autoclaved with caps off, so to ensure sterility we've stored them on ethanol-soaked paper.
-#Inoculated 5mL LB Miller medium and grow overnight at 37&deg;C with rotation. <br> Austin Che calls for LB Lennox to lessen salt content, but we have only LB Miller and have attempted to use that; we'll post an update!
+#Prechilled the rotor at 4&deg;C.
+** All tubes and pipets will be chilled tomorrow morning upon arrival.
+#Inoculated 5mL LB Miller medium and placed in incubator for overnight growth at 37&deg;C with rotation. <br> Austin Che calls for LB Lennox to lessen salt content. We had only LB Miller at the time of inoculation and have attempted to use that; we'll post an update!  For tomorrow's 450mL culture, we've prepared 500mL of LB Lennox.