IGEM:UC Berkeley/2006/Gel extraction

From OpenWetWare

< IGEM:UC Berkeley/2006
Revision as of 17:20, 8 August 2006 by Bryanh (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
  1. cut out band minimizing extra gel matter.
  2. put in ependorf tube and add 600uL of QG.
  3. vortex until the gel has dissovled.
  4. add 100uL of isopropanol alcohol.
  5. mix
  6. pour into the minelute column. this has a dark purple band around the resin which distinguishes it from the miniprep columns.
  7. spin through. and disgard waste.
  8. the book says to run more QG through but i dont because it doesnt do anything.
  9. wash with 750uL PE buffer.
  10. spin for two minutes to dry.
  11. elute with 2mM Tris buffer.
Personal tools