IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/25: Difference between revisions
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(Autocreate 2011/08/25 Entry for IGEM:UNAM-Genomics_Mexico/2009/Notebook/H2_Pae) |
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== | ==An error== | ||
Today the team realized that the standardization had an error, because a fragment neccesary was absent in our pBBR1MCS-5 standard plasmid. this is because when we didn't amplify the full standard region form the pSB1T3 plasmid (which was inserted into pBBR1MCS-5), due to an error designing the forward primer. I re-designed it and will repeat all the experiment, the new primer is: | |||
>RFP-UP-Ok///melting60.8°C | |||
CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT | |||
Revision as of 10:58, 27 August 2011
 iGEM Project name 1
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An errorToday the team realized that the standardization had an error, because a fragment neccesary was absent in our pBBR1MCS-5 standard plasmid. this is because when we didn't amplify the full standard region form the pSB1T3 plasmid (which was inserted into pBBR1MCS-5), due to an error designing the forward primer. I re-designed it and will repeat all the experiment, the new primer is: >RFP-UP-Ok///melting60.8°C CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT
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