IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:


>RFP-UP-Ok///melting60.8°C
>RFP-UP-Ok///melting60.8°C
CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT
CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT


Revision as of 10:58, 27 August 2011

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

An error

Today the team realized that the standardization had an error, because a fragment neccesary was absent in our pBBR1MCS-5 standard plasmid. this is because when we didn't amplify the full standard region form the pSB1T3 plasmid (which was inserted into pBBR1MCS-5), due to an error designing the forward primer. I re-designed it and will repeat all the experiment, the new primer is:

>RFP-UP-Ok///melting60.8°C

CGG GCC CGA GTG CCA CCT GAC GTC TAA GAA ACC ATT ATT AT




Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:UNAM-Genomics_Mexico/2009/Notebook/H2_Pae/2011/08/25&oldid=531987"