IGEM:UNAM-Genomics Mexico/2009/Notebook/iGEM 2011 H2/2011/06/14: Difference between revisions
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*The control was prepared using the same quantites of each reagent except that instead of adding 2 µL of DNA, I added 34 µL of H2O. | |||
3. To asses the size of the amplified DNA fragment the PCR products were run in a 2% agarose gel at 100 V for 1 hour. The gel was later stained with ethidium bromide. The results are pictured below. | |||
3. To asses the size of the amplified DNA fragment the PCR products were run in a 2% agarose gel at 100 V for 1 hour. I loaded 5 µL of each PCR product + 1 µL of die. The gel was later stained with ethidium bromide. The results are pictured below. | |||
Revision as of 00:58, 15 June 2011
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Amplification of the promoter region of the gene nifH of 'R. etliObjective The goal was to amplify the promoter region of the gene nifH of R. etli via PCR. The final result is a 246 bp segment containg the iGEM preffix, the promoter region mentioned above, and the iGEM suffix for further manipulation of the promoter region.
In order to obtain 500 µl of the oligos in the desired concentration, I diluted the oligos as follows:
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