IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/13: Difference between revisions
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*Make ligation for promoter and double terminator.(Mariana) | *Make ligation for promoter and double terminator.(Mariana) | ||
*Get Luciferase, get mutant and clone it. (Mariana). | *Get Luciferase, get mutant and clone it. (Mariana). | ||
*Primers used for blue promoter: BBa_K238013 | *Primers used for blue promoter: [[Link:partsregistry.org/Part:BBa_K238013:Experience|BBa_K238013]] | ||
==Modeling== | ==Modeling== |
Revision as of 17:03, 15 May 2010
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13th may 2010WetLabExtraction of genomic DNA for blue promoter is done, but we don´t have amplified. Goals for next week
ModelingWe´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin. =Goals for next week
Required data
propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration. TRpR → aλ[P1] Promoter of Blue receptor → bλ[P2] Where λ is light. Ps, concentrations.
General
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