IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12: Difference between revisions
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1. | 1. Restriction digest of pTrc99A-AID with HindIII and NcoI. | ||
2. DNA electrophoresis and isolation of DNA encoding AID from proper band. | 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. | ||
3. | 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). | ||
4. DNA electrophoresis and isolation of pMPM-T5 | 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. | ||
5. DNA ligation (pMPM-T5+AID vector) | |||
[[Image:UW_AID.jpg|350px]] | |||
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Revision as of 11:36, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. Restriction digest of pTrc99A-AID with HindIII and NcoI. 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. 5. DNA ligation (pMPM-T5+AID vector)
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