IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/23: Difference between revisions
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1. Digestion of pMPM-T5 with | 1. Digestion of pMPM-T5 with NcoI and XbaI | ||
2. Digestion of PCR product for translation fusion (AID+T7 RNA-polimerase) with | 2. Digestion of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI | ||
3. Clean-up of digested products from p.1 and p.2 | 3. Clean-up of digested products from p.1 and p.2 | ||
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5. Transformation of E.coli TOP10 with ligation product | 5. Transformation of E.coli TOP10 with ligation product | ||
6. Digestion of pMPM-T5+AID with | 6. Digestion of pMPM-T5+AID with HindIII and SalI | ||
7. Digestion of PCR product for transcription fusion (T7 RNA-polimerase) with | 7. Digestion of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI | ||
8. Clean-up of digested products from p.6 and p.7 | 8. Clean-up of digested products from p.6 and p.7 | ||
Revision as of 01:48, 11 June 2008
 University of Warsaw iGEM Team 2008
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1. Digestion of pMPM-T5 with NcoI and XbaI 2. Digestion of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI 3. Clean-up of digested products from p.1 and p.2 4. Ligation of digested products from p.1 and p.2 5. Transformation of E.coli TOP10 with ligation product 6. Digestion of pMPM-T5+AID with HindIII and SalI 7. Digestion of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI 8. Clean-up of digested products from p.6 and p.7 9. Ligation of digested products from p.6 and p.7 10. Transformation of E.coli TOP10 with ligation product | |
