Jacobs:Back Door: Difference between revisions
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*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | *[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | ||
*[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]] | *[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]] | ||
*[[Jacobs: In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | *[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | ||
*[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | *[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | ||
*[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]] | *[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]] | ||
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*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]] | *[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]] | ||
*[[Jacobs:Protocol Printers | Printers]] | *[[Jacobs:Protocol Printers | Printers]] | ||
*[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | |||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
Revision as of 10:40, 24 February 2011
Useful Links / Meetings
Equipment and Schedules
Protocols
Research Protocols
- Safety
- Freezing and Thawing Cells
- Actin Staining
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis
- Total Protein Isolation Using RIPA Lysis Buffer
- Transfecting NIH 3T3s with GFP-Actin
- 10 bp DNA Ladder
- Collagen Gel Contraction 2
- Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis
- Loading Mice
- OFF (Large Chambers)
- Flow chamber calibration
- siRNA transfection
- Separating and Tagging of Mice
- Genotyping Tailsnips
- Tubulin Immunostaining Protocol
- Confocal Imaging of Flow
- Cell Culture numbers
- Troubleshooting Cell Culture
- Lecia MP Microscope Protocol
- In vivo microCT scanning protocol for live mice
- Isolation of Primary Bone Cells
- Jacobs: Cell Culture of MLOY-4 Osteocyte Cells
- Jacobs: Cell Culture of IMCD Kidney cells
- Autoclave
- Alkaline Phosphatase Assay
- Neon Transfection
- Coating Slides with Fibronectin
- Osteocyte Markers
- Printers
- Immunostaining for TRPV4 and PC
