Jacobs:Back Door: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
An M. Nguyen (talk | contribs) |
An M. Nguyen (talk | contribs) |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 38: | Line 38: | ||
**[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | **[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | ||
**[[Jacobs:Confocal Imaging of Flow| Confocal imaging of primary cilia under flow]] | **[[Jacobs:Confocal Imaging of Flow| Confocal imaging of primary cilia under flow]] | ||
*Immunocytochemistry | *Immunocytochemistry | ||
**[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | **[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | ||
| Line 44: | Line 45: | ||
**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]] | **[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]] | ||
*Assays | *Assays | ||
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein Content]] | **[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein Content]] | ||
**[[Jacobs:Protocol Real-Time PCR|Real-Time RT qPCR]] | **[[Jacobs:Protocol Real-Time PCR|Real-Time RT qPCR]] | ||
**[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]] | **[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]] | ||
| Line 60: | Line 61: | ||
* Mice | * Mice | ||
**[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | **[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | ||
**[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]] | **[[Jacobs:Genotyping Tailsnips|Genotyping by qPCR]] | ||
**[[Media:Genotyping Tailsnips.doc | Genotyping by agarose gel]] | |||
**[[Jacobs:Protocol Loading Mice|Loading Mice]] | **[[Jacobs:Protocol Loading Mice|Loading Mice]] | ||
**[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | **[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | ||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]] | |||
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]] | *[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]] | ||
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]] | *[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]] | ||
| Line 70: | Line 73: | ||
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]] | *[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]] | ||
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]] | *[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]] | ||
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]] | *[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]] | ||
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]] | *[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]] | ||
Latest revision as of 12:49, 2 December 2014
Useful Links / Meetings
Protocols
Research Protocols
- General
- Cells
- Flow
- Immunocytochemistry
- Assays
- Gels & blots
- Protein
- DNA & RNA
- Mice
Teaching Protocols
- Pipetting Exercise
- Adipocyte Differentiation
- Oil Red O Staining
- RNA Agarose Gel
- Collagen Gel Contraction
- Collagen Gel Contraction 2
- Total Protein Isolation using RIPA buffer
- Total Protein Isolation using RIPA buffer 2
- RNA Sample Loading Buffer
