Jacobs:Back Door: Difference between revisions

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==Protocols==
==Protocols==
===Research Protocols===
===Research Protocols===
*[[Jacobs:Protocol Safety|Safety]]
*General
*[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]]
**[[Jacobs:Protocol Printers | Printers]]
*[[Jacobs:Protocol Actin Staining|Actin Staining]]
**[[Jacobs:Protocol Safety|Safety]]
**[[Jacobs:Protocol Autoclave | Autoclave]]
*Cell culture
**[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]]
**[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]]
**[[Jacobs: Cell Culture of IMCD Kidney cells]]
**[[Jacobs:Protocol Cell Culture of MDCK cells | Cell Culture of MDCK cells]]
**[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
**[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
**[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]]
**[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]]
**[[Jacobs:Protocol siRNA transfection|siRNA transfection]]
**[[Jacobs:Protocol Neon Transfection | Neon Transfection]]
**[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
**[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
*Flow
**[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
**[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]]
**[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]]
*Immunocytochemistry
**[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]]
**[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]]
**[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]]
**[[Jacobs:Protocol Actin Staining|Actin Staining]]
**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]]
*Gels & blots
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]]
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis]]
*[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis]]
*[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
* Mouse work
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis]]
**[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]]
*[[Jacobs:Protocol Loading Mice|Loading Mice]]
**[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]]
*[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
**[[Jacobs:Protocol Loading Mice|Loading Mice]]
*[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]]
**[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]]
*[[Jacobs:Protocol siRNA transfection|siRNA transfection]]
 
*[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]]
 
*[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]]
 
*[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]]
 
*[[Jacobs:Confocal Imaging of Flow| Confocal Imaging of Flow]]
*[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
*[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
*[[Jacobs: Leica MP Microscope Protocol| Lecia MP Microscope Protocol]]
*[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]]
*[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]]
*[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]]
*[[Jacobs: Cell Culture of IMCD Kidney cells]]
*[[Jacobs:Protocol Cell Culture of MDCK cells | Cell Culture of MDCK cells]]
*[[Jacobs:Protocol Autoclave | Autoclave]]
*[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase Assay]]
*[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase Assay]]
*[[Jacobs:Protocol Neon Transfection | Neon Transfection]]
 
*[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]]
 
*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]]
*[[Jacobs:Protocol Osteocyte Markers|Osteocyte Markers]]
*[[Jacobs:Protocol Printers | Printers]]
 
*[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]]
*[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]]


===Teaching Protocols===
===Teaching Protocols===
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein]]
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein]]
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]]
*[[Jacobs:Protocol RNA Agarose Gel|RNA Agarose Gel]]
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]


*[[Jacobs:Protocol Real-Time PCR|Real-Time PCR]]
*[[Jacobs:Protocol Real-Time PCR|Real-Time PCR]]

Revision as of 12:09, 2 December 2014



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