Jacobs:Back Door: Difference between revisions
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**[[Jacobs:Protocol Autoclave | Autoclave]] | **[[Jacobs:Protocol Autoclave | Autoclave]] | ||
**[[Jacobs: Leica MP Microscope Protocol| Leica MP Microscope]] | **[[Jacobs: Leica MP Microscope Protocol| Leica MP Microscope]] | ||
* | *Cells | ||
** | **Culture | ||
**[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells | Culture of MLO-Y4s]] | ***[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells | Culture of MLO-Y4s]] | ||
**[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]] | ***[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]] | ||
**[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]] | ***[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]] | ||
**[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Culture of 3T3 Fibroblasts]] | ***[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Culture of 3T3 Fibroblasts]] | ||
**[[Jacobs: | ***[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | ||
**[[Jacobs:Cell Culture | ***[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | ||
**[[Jacobs:Protocol Coating Slides with Fibronectin | Coating | ***[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]] | ||
**Transfection | |||
***[[Jacobs:Protocol siRNA transfection|siRNA transfection]] | |||
***[[Jacobs:Protocol Neon Transfection | Neon Transfection]] | |||
***[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]] | |||
**[[Jacobs:Protocol Coating Slides with Fibronectin | Coating with Fibronectin]] | |||
**[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | **[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | ||
**[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]] | **[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]] | ||
**[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]] | **[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]] | ||
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***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]] | ***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]] | ||
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]] | ***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]] | ||
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]] | ***[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]] | ||
* | * Mice | ||
**[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | **[[Jacobs:Protocol Separating and Tagging of Mice|Separating and Tagging of Mice]] | ||
**[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]] | **[[Jacobs:Genotyping Tailsnips|Genotyping Tailsnips]] | ||
Revision as of 12:23, 2 December 2014
Useful Links / Meetings
Protocols
Research Protocols
- General
- Cells
- Flow
- Immunocytochemistry
- Assays
- BCA Total Protein Content
- Gels & blots
- Protein
- DNA & RNA
- Mice
Teaching Protocols
- Adipocyte Differentiation
- Oil Red O Staining
- RNA Agarose Gel
- Collagen Gel Contraction
- Collagen Gel Contraction 2
- Pipetting Exercise
- Total Protein Isolation using RIPA buffer
- Total Protein Isolation using RIPA buffer 2
- RNA Sample Loading Buffer
