Jacobs:Back Door
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Useful Links / Meetings
Protocols
Research Protocols
- General
- Cell culture
- Freezing and Thawing Cells
- Culture of MLO-Y4s
- Culture of IMCDs
- Culture of MDCKs
- Culture of 3T3 Fibroblasts
- Troubleshooting Cell Culture
- Cell Culture numbers
- Coating Slides with Fibronectin
- Isolation of Primary Bone Cells
- siRNA transfection
- Neon Transfection
- Transfecting NIH 3T3s with GFP-Actin
- Total Protein Isolation Using RIPA Lysis Buffer
- Total RNA Isolation using Tri Reagent
- Flow
- Immunocytochemistry
- Assays
- BCA Total Protein Content
- Gels & blots
- Protein
- DNA & RNA
- 10 bp DNA Ladder
- Mouse work
Teaching Protocols
- Adipocyte Differentiation
- Oil Red O Staining
- RNA Agarose Gel
- Collagen Gel Contraction
- Collagen Gel Contraction 2
- Pipetting Exercise
- Total Protein Isolation using RIPA buffer
- Total Protein Isolation using RIPA buffer 2
- RNA Sample Loading Buffer