Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/10/21: Difference between revisions

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(Autocreate 2010/10/21 Entry for Jasieniuk:Notebook/Apomixis_in_hybrid_Rubus)
 
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==Entry title==
==Formamide plates==
* Insert content here...
* Prepped formamide plates for RUB101011 and RUB101012
* Used leftover formamide mix from October 4 on the second half of RUB101012
* Added 9 ul GS500 to each of three 1 mL tubes of formamide
* 19 ul formamide mix + 1 ul diluted PCR product
* Spun down plates and denatured them, then stored at 4 degrees C while waiting for sequencer batteries to be replaced.





Revision as of 13:51, 25 October 2010

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Formamide plates

  • Prepped formamide plates for RUB101011 and RUB101012
  • Used leftover formamide mix from October 4 on the second half of RUB101012
  • Added 9 ul GS500 to each of three 1 mL tubes of formamide
  • 19 ul formamide mix + 1 ul diluted PCR product
  • Spun down plates and denatured them, then stored at 4 degrees C while waiting for sequencer batteries to be replaced.