Jeff Tabor/MEGAWHOP: Difference between revisions
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(New page: == PCR setup == *120ng template plasmid *500ng Megaprimer *Pfu Ultra II == Cycling conditions == 95C, 2' <br> 95C, 20" <br> 55C, 20" <br> 68C, 6-12' <br> 25x <br> == Transformation == *D...) |
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Revision as of 16:20, 20 November 2008
PCR setup
- 120ng template plasmid
- 500ng Megaprimer
- Pfu Ultra II
Cycling conditions
95C, 2'
95C, 20"
55C, 20"
68C, 6-12'
25x
Transformation
- DpnI, 1-2h
- Transform 0.5-2uL into 50uL chemically competent E.coli
