KAD072106-Construction of pSB1A2-J01129-F1610: Difference between revisions
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Digest pSB1AK3-F1610 (XbaI/AlwnI, Neb2, small-1430bp)<br> | Digest pSB1AK3-F1610 (XbaI/AlwnI, Neb2, small-1430bp)<br> | ||
Paste into pSB1A2-J01129 (SpeI/PstI, Neb2, large-1689bp)<br> | Paste into pSB1A2-J01129 (SpeI/PstI, Neb2, large-1689bp)<br> | ||
Product is pSB1A2-J23029.<br> | |||
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Digest pSB1Ak3-F1610 (XbaI/PstI, Neb2, Small-820bp)<br> | |||
Paste into pSB1A2-J01129 (SpeI/PastI, Neb2, Large-2221bp)<br> | |||
Product is pSB1A2-J23029.<br> | |||
Latest revision as of 11:07, 27 July 2006
[EcoRI][TT][Ptet][RBS][XbaI][RFP][TT]
Digest pSB1AK3-F1610 (XbaI/AlwnI, Neb2, small-1430bp)
Paste into pSB1A2-J01129 (SpeI/PstI, Neb2, large-1689bp)
Product is pSB1A2-J23029.
Digest pSB1Ak3-F1610 (XbaI/PstI, Neb2, Small-820bp)
Paste into pSB1A2-J01129 (SpeI/PastI, Neb2, Large-2221bp)
Product is pSB1A2-J23029.
