KAD072106-Construction of pSB1A2-J01129-F1610
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[EcoRI][TT][Ptet][RBS][XbaI][RFP][TT]
Digest pSB1AK3-F1610 (XbaI/AlwnI, Neb2, small-1430bp)
Paste into pSB1A2-J01129 (SpeI/PstI, Neb2, large-1689bp)
Product is pSB1A2-J23029.
Digest pSB1Ak3-F1610 (XbaI/PstI, Neb2, Small-820bp)
Paste into pSB1A2-J01129 (SpeI/PastI, Neb2, Large-2221bp)
Product is pSB1A2-J23029.
