KAD072406-Construction of pSB1A2-J01129-J01006: Difference between revisions
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[pK3(presumably on pSB1A2]+[key3]<br> | [pK3(presumably on pSB1A2)]+[key3]<br> | ||
Digest pSB1A2-J01006 [AlwnI/SpeI, Neb2, Large-1590bp]<br> | Digest pSB1A2-J01006 [AlwnI/SpeI, Neb2, Large-1590bp]<br> | ||
Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]<br> | Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]<br> | ||
Product is pSB1A2-J23025<br> | Product is pSB1A2-J23025<br> | ||
this construction has been abandoned because it turns out that we dont have the basic part for key3. the construction file here describes the construction of [pK3][Ptet][key3] which is stupid. | |||
Latest revision as of 14:00, 3 August 2006
[pK3(presumably on pSB1A2)]+[key3]
Digest pSB1A2-J01006 [AlwnI/SpeI, Neb2, Large-1590bp]
Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]
Product is pSB1A2-J23025
this construction has been abandoned because it turns out that we dont have the basic part for key3. the construction file here describes the construction of [pK3][Ptet][key3] which is stupid.
