KAD072406-Construction of pSB1A2-J01129-J01006
[pK3(presumably on pSB1A2)]+[key3]
Digest pSB1A2-J01006 [AlwnI/SpeI, Neb2, Large-1590bp]
Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]
Product is pSB1A2-J23025
this construction has been abandoned because it turns out that we dont have the basic part for key3. the construction file here describes the construction of [pK3][Ptet][key3] which is stupid.