KAD072406-Construction of pSB1A2-J01129-J01006

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[pK3(presumably on pSB1A2)]+[key3]
Digest pSB1A2-J01006 [AlwnI/SpeI, Neb2, Large-1590bp]
Paste into pSB1A2-J01129 [AlwnI/XbaI, Neb2, Small-710bp]
Product is pSB1A2-J23025

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