KP Ramirez Week 11: Difference between revisions
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Experimental --> purify RNA --> Label --> Cy5 Red | Experimental --> purify RNA --> Label --> Cy5 Red | ||
With human samples its more difficult then model organisms. With model organisms there are great lengths to show they have the same genetic background. For human samples, all individuals are unique, genes have variations in them making them different from person to person. There is no model control environment. When talking about what tissue is used it becomes more complicated. '''For example, using blood, organs, skin, on our chips were biopsy taken from men that were taken during treatment.''' | |||
{{Kevin A Paiz-Ramirez}} | {{Kevin A Paiz-Ramirez}} | ||
Revision as of 08:33, 6 April 2010
Biological replicates & technical replicates
Compare what they say in their methods with the outline that is on the wiki, did they detail all of their steps or did they leave their information out.
Figure out where the source of the data came from, match the columns of the data that appear on the excel spreadsheet and how they relate to the paper, see what samples correspond to the paper.
control --> purify RNA --> Label --> cy3 Green
Experimental --> purify RNA --> Label --> Cy5 Red
With human samples its more difficult then model organisms. With model organisms there are great lengths to show they have the same genetic background. For human samples, all individuals are unique, genes have variations in them making them different from person to person. There is no model control environment. When talking about what tissue is used it becomes more complicated. For example, using blood, organs, skin, on our chips were biopsy taken from men that were taken during treatment.
Journal Assignments
