Klapperich Lab:Notebook/Lab Meeting Notes/2008/11/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 13: Line 13:
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
* Courier service!<br>
* Courier service! Sample transport set up. <br>
* Plaque assay status?<br>
* Plaque assay status?<br>
* Cathie Submitted the progress report to INSPIR. <br>
* Mark - status of fixture? <br>
<br>
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
† SEPSIS Project - CIMIT Bird Flu <br>
* Run E. coli RT-PCR using primers for a genomic target.<br>
* Cathie working on paper.<br>
* Report DNA amounts along with the RT-PCR data for the Sepsis paper.<br>
<br>
<br>
†RNA project
†RNA project
* Jeff Braman will visit in early Dec. <br>
* Jeff Braman will visit in early Dec. <br>
* Do dilutions. Send to Bioanalyzer. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark finish control circuit <br>  
* Mark- Control circuit? <br>  
* Bacteria Experiment. Failed first time. Any progress? <br>
* Bacteria Experiment - Update from Jane and Jaephil? <br>
* Jane, Cathie, Larry, Ranjith met again. JZ will try process with teflon substrates.  <br>
* Substrates - JZ to try with more gold and do all SEM/sample testing.  <br>
<br>
<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Waiting for fixture still from Melissa. Hopefully this week. UPDATE? <br>
* Status from Mark? <br>
* Received schematic and quote.  Frank met with Dr. Do to go over schematic and see what's next.  Met with Sonali (3 Nov) to better delineate system requirements, will meet with Mark to coordinate fixture/pressure-driven flow system interfacing before submitting revised specs to Superior Fluid Solutions.<br>
* Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.<br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
* Update? <br>
* Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips. <br>
* Alex working on more toluene bonding issues. <br>
* <br>
* Try Ticona material. (HM, JD, AG) <br>
* Still Still Still troubleshooting '''??'''<br>
<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Mask writer's calibration was done. <br>
* Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine. - UPDATE?<br>
* Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine.<br>
* Preliminary E. coli experiment is ongoing. UPDATE? <br>
* Preliminary E. coli experiment is ongoing. <br>
* Try DAPI bacteria stain to visualize e-coli soon. UPDATE? <br>
* Try DAPI bacteria stain to visualize e-coli soon. <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
Line 51: Line 47:
<br>
<br>
† PCR <br>
† PCR <br>
* QQ gel issues. Resolved??  <br>
* QQ gel issues. Stop using ABI flu probe that QQ ordered. Try Cathie's old assay before designing a new one. Run gels. <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE? <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. <br>'''
* Qingqing has to do experiments with MinCheol's designs. AS SOON AS CLEANING CONTEROLS ARE IN PLACE. <br>
* MinCheol's desgins. UPDATE? <br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
* Mincheol will work on DNA migration simulation after IMECE 2008.<br>
<br>
<br>
Line 61: Line 57:
<br>
<br>
† Senior project <br>
† Senior project <br>
* Megan ordering oligos and primers for the RCA. Quote. Check with Heiko on his sources. <br>
* Need updates from both Senior project students in bullet form. <br>
* Haven't seen Teddy yet. What is up with him? <br>
* Emailed Teddy. <br>
<br>
<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
Line 71: Line 67:
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* Ongoing. More concentrations. . <br>
* Ongoing. More concentrations. <br>
* Pressure testing on these channels when they are flowing. <br>
* Pressure testing on these channels when they are flowing. <br>


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 11:36, 10 November 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

11 November 2008 Lab Meeting Agenda

† Hussam's research report today.

† Announcements

  • Your personal priorities have been emailed to you by me. Please take note.


† Flu R01

  • Courier service! Sample transport set up.
  • Plaque assay status?
  • Mark - status of fixture?


† SEPSIS Project - CIMIT Bird Flu

  • Cathie working on paper.


†RNA project

  • Jeff Braman will visit in early Dec.
  • Do cDNA prep. Spec, gels, send to Jeff B.

† COBRA

  • Mark- Control circuit?
  • Bacteria Experiment - Update from Jane and Jaephil?
  • Substrates - JZ to try with more gold and do all SEM/sample testing.


† Valve Array/Valve building (FJ)

  • Status from Mark?
  • Frank should talk with Alex, Mark, and Jaephil and make sure we are not duplicating efforts/making something that is too big for portability.


† Fraunhofer: LOAC

  • Primer/dimer issue. Talked with QQ. We do not appear to have this problem with our chips.


† Biointerfaces group

  • Paul Mak told that Mask aligner at Photonics center has UV filter. Try that machine. - UPDATE?
  • Preliminary E. coli experiment is ongoing. UPDATE?
  • Try DAPI bacteria stain to visualize e-coli soon. UPDATE?


† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • QQ gel issues. Stop using ABI flu probe that QQ ordered. Try Cathie's old assay before designing a new one. Run gels.
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - Will try after fixes and report next week. UPDATE?

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • MinCheol's desgins. UPDATE?
  • Mincheol will work on DNA migration simulation after IMECE 2008.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Need updates from both Senior project students in bullet form.
  • Emailed Teddy.


† F31: Cochlea

  • Received paper edits from Doug.
  • Make changes and give to Cathie ASAP.
  • Journal chosen?
  • Need to order Alpha-Tectorin protein.


† Silica Optimization (Lambda):

  • Ongoing. More concentrations.
  • Pressure testing on these channels when they are flowing.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Klapperich_Lab:Notebook/Lab_Meeting_Notes/2008/11/11&oldid=259840"