Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/09: Difference between revisions

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† Announcements<br>
† Announcements<br>
* Meeting on 12/16.
* Meeting on 12/16. <br>
* No meeting on 12/23 <br>
* No meeting on 12/23 <br>
* Meeting Time for Spring Semester (starting week of 1/16). Put your NOT OPEN times here.<br>
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
* First samples taken starting next week, 11/19. <br>
* First samples ? <br>
* Sample training 9:30am Wed. <br>
* Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml <br>
* Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml <br>
* Mark trained for real to make SU molds. Trained to make the monoliths. <br>
* Alex needs to be trained to make SU molds. Trained to make the monoliths. <br>
* Hussam: Flu fixture will be ready 11-19-08 (tomorrow)- 6 channel fixture (2). <br>
* Hussam: Flu fixture done. <br>
*  
*  
<br>
<br>
† SEPSIS Project - CIMIT Bird Flu <br>
† SEPSIS Project - CIMIT Bird Flu <br>
* Cathie STILL working on paper.<br>
* Sonali  working on first draft of new version paper.<br>
<br>
<br>
†RNA project
†RNA project
* Jeff Braman will visit December 12th <br>
* Jeff Braman will visit December 12th. 9:30am Visit to Cathie's lab and lab tour. Meetings starting at 10am in Fraunhofer. Sonali and Cathie to attend. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark- Control circuit? <br>  
* Mark- Control circuit?  - will this be done by January?<br>  
* Bacteria Experiment - Changing channel size. <br>
* Evap quantification experiments ongoing. (JD, JZ)<br>
* Substrates -SEM down. <br>
* Substrates (JZ)<br>
<br>
<br>
† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Valves backorderd until 24th. <br>
* Turn over platform to Teddy <br>
* <br>
* <br>
* <br>
* <br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
* <br>
* So close!<br>
<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Mask  made. Chrome mask. <br>
* <br>
* E. Coli experiments with fluorescence? <br>
* E. Coli experiments with fluorescence. <br>
* Aim for fluor data for 6th. <br>
* Strobe experiments? Jason cannot do them, perhaps MK can. <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
* Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09<br>
* First pages of first IRB started last week. Target date 1/1/09<br>
* STAR-CD Renewal - January ($2K)<br>
* STAR-CD Renewal - January ($2K)<br>
<br>
<br>
† PCR <br>
† PCR <br>
* Cathie's flu assay has nonspecific products, too. <br>
* <br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Look into Melting temp analysis on the 7300  - Done. what is contaminated? <br>
* Look into Melting temp analysis on the 7300  - Done. what is contaminated? <br>
Line 65: Line 65:
<br>
<br>
† Senior project <br>
† Senior project <br>
* Megan has mask designs. CHECK WITH HER. <br>
* <br>
* Depth of channels, check with JD and Sonali, because Sonali will be able to tell you how much assay volume. 100 microns. <br>
* <br>
* Mark will help/learn SU8 step. <br>
* Teddy doing off chip controls work.  <br>
<br>
<br>
<br>
<br>
† F31: Cochlea <br>
† F31: Cochlea <br>
* Submit paper. <br>
* Paper submitted.<br>
*  
*  
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* UPDATE 11-18-08 <br>
* <br>
* De-inhibit Methyl Methacrylate (100ml), put in fridge, make new columns. (today) <br>
* Ongoing. More concentrations. saved samples in -20 until pellet paint figured out <br>
* Repeating experiments this week with activated MMA and collecting more samples <br>
* Plates used for Pico Green are not sterile ?? NOT LIKELY. <br>
* Pico Green assay figured out and standards working.<br>
* Pellet paint: Even (-) ctrl form a pellet , after 100% ethanol wash pellet is detached.<br>
* try std. etoh protocol. <br>
*  
*  


Revision as of 14:08, 8 December 2008

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9 December 2008 Lab Meeting Agenda

† Stephanie's research report today.

† Announcements

  • Meeting on 12/16.
  • No meeting on 12/23
  • Meeting Time for Spring Semester (starting week of 1/16). Put your NOT OPEN times here.


† Flu R01

  • First samples ?
  • Plaque Assay - first samples today. Ref stock 2x10^4 pfu/ml
  • Alex needs to be trained to make SU molds. Trained to make the monoliths.
  • Hussam: Flu fixture done.


† SEPSIS Project - CIMIT Bird Flu

  • Sonali working on first draft of new version paper.


†RNA project

  • Jeff Braman will visit December 12th. 9:30am Visit to Cathie's lab and lab tour. Meetings starting at 10am in Fraunhofer. Sonali and Cathie to attend.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit? - will this be done by January?
  • Evap quantification experiments ongoing. (JD, JZ)
  • Substrates (JZ)


† Valve Array/Valve building (FJ)

  • Turn over platform to Teddy



† Fraunhofer: LOAC

  • So close!


† Biointerfaces group


  • E. Coli experiments with fluorescence.
  • Strobe experiments? Jason cannot do them, perhaps MK can.


† CIMIT- Colson Grant

  • First pages of first IRB started last week. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR


  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - Done. what is contaminated?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit.

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture.

  • Design new lambda phage assay with longer product.
  • What do we need to finish the paper?


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project





† F31: Cochlea

  • Paper submitted.


† Silica Optimization (Lambda):



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