Klapperich Lab:Notebook/Lab Meeting Notes/2008/12/16: Difference between revisions

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==16 December 2008 Lab Meeting Agenda==
==16 December 2008 Lab Meeting Agenda==


* check with b&G about compressed air and vacuum?
* Check with B&G about compressed air and vacuum?


† Announcements<br>
† Announcements<br>
* No meeting on 12/23 or 12/30 <br>
* No meeting on 12/23 or 12/30 <br>
* Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. See Lab Calendar for dates.
* Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates.
Cathie T/TH 12-2pm.<br>
 
Jane T 3-5 <br>
Nathan F 10-12 <br>
Megan Mon (9am-6pm), Tues (1-4pm), Wed (9am-2pm) <br>
Frank has OPEN M 12-4PM, T/TH before 10AM, W-F after 3PM, F 11AM-1PM  <br>
Hussam Mon, (10-2 pm),  (Wed 10-5), (Fri 10-12) ,(Tue Thur 10-12  pm) <br>
Sonali MWF 11am -1pm.
Mincheol Mon-Fri 9am-1pm
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
* First samples ? <br>
* First samples? <br>
* Plaque Assay <br>
* Plaque Assay. <br>
* Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09). <br>
* Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09). <br>
* Hussam: Flu fixture done. <br>
*
<br>
<br>
† SEPSIS Project <br>
† SEPSIS Project <br>
* Sonali  working on first draft of new version paper by 12/25. Journal?<br>
* Sonali  working on first draft of new version paper by 12/25. Journal?<br>
* Hussam working on Lambda phage data. <br>
<br>
<br>
†RNA project
†RNA project
* Jeff Braman will visit December 12th. 9:30am Visit to Cathie's lab and lab tour. Meetings starting at 10am in Fraunhofer. Sonali and Cathie to attend. <br>
* Report to Jeff from Cathie. <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
* Do cDNA prep. Spec, gels, send to Jeff B. Shipping? <br>
  <br>
  <br>
† COBRA <br>
† COBRA <br>
* Mark- Control circuit - This will be done by January.<br>  
* Mark- Control circuit - This will be done by January.<br>  
* Evap quantification experiments ongoing. Still working on live/dead assay. (JD, JZ)<br>
* Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)<br>
- problems: dead but green in the end <br>
- problems: dead but green in the end <br>
- called tech support. <br>
- called tech support. <br>
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. <br>
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. <br>
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute <br>  
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute <br>  
- try running the experiment first, then running the live/dead dye through second. <br>


* Substrates (JZ)<br>
* Substrates (JZ)<br>
Line 53: Line 46:
- needs much higher particle/bacteria ratio <br>
- needs much higher particle/bacteria ratio <br>
- experiments outside the channel first? <br>
- experiments outside the channel first? <br>
* Prospectus framework/discussion: target the end of this week <br>
* Prospectus framework/discussion: target the end of this week 1/23/09. <br>
<br>
<br>


† Valve Array/Valve building (FJ) <br>
† Valve Array/Valve building (FJ) <br>
* Turn over platform to Teddy <br>
* Turn over platform to Teddy <br>
* <br>
* TEDDY needs to meet with Mark.<br>
* <br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
* So close!<br>
* So so close!<br>
<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* <br>
* <br>
* E. Coli experiments with fluorescence. <br>
* Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. <br>
* Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. <br>
* Made new design for trapper. <br>
* Made new design for trapper. <br>
Line 72: Line 63:
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
* First pages of first IRB started last week.  Target date 1/1/09<br>
* IRB still going/ revisions by tomorrow to Ian.  Target date 1/1/09<br>
* STAR-CD Renewal - January ($2K)<br>
* STAR-CD Renewal - January ($2K)<br>
<br>
<br>
† PCR <br>
† PCR <br>
* <br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?<br>
* Look into Melting temp analysis on the 7300  - Done. what is contaminated? <br>
* Look into Melting temp analysis on the 7300  - Done. what is contaminated? <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD <br>
* Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD <br>
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD<br>'''
'''* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD<br>'''
* Try microscope for looking a fluor. <br>
* Try microscope for looking a fluor. Learn how to look at the data in Image J <br>
* 52, 60, 65 C repeats. <br>
* 52, 60, 65 C repeats. <br>
* <br>
* <br>
Line 92: Line 82:
* <br>
* <br>
† F31: Cochlea <br>
† F31: Cochlea <br>
* Paper submitted.<br>
* Set up adviser meeting Jan <br>
* set up adviser meeting Jan <br>
* Post Gent vs. Pre gent - 4 proteins. 3/4 no change.  <br>
<br>
<br>
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* inhibition infor to Paul. <br>
* Update by 12/24. <br>
* Replot the data so I can read it. ASAP <br>
* Do three elutions. <br>
* Do three elutions. <br>
* Next E. coli. <br>
* Next E. coli. <br>

Revision as of 11:17, 16 December 2008

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16 December 2008 Lab Meeting Agenda

  • Check with B&G about compressed air and vacuum?

† Announcements

  • No meeting on 12/23 or 12/30
  • Meeting Time for Spring Semester is THURSDAYS 3-5pm ROOM 705. Stating 1/8/09. See Lab Calendar for dates.


† Flu R01

  • First samples?
  • Plaque Assay.
  • Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09).


† SEPSIS Project

  • Sonali working on first draft of new version paper by 12/25. Journal?
  • Hussam working on Lambda phage data.


†RNA project

  • Report to Jeff from Cathie.
  • Do cDNA prep. Spec, gels, send to Jeff B. Shipping?

† COBRA

  • Mark- Control circuit - This will be done by January.
  • Evap quantification experiments ongoing. Still working on live/dead assay.(JD, JZ)

- problems: dead but green in the end
- called tech support.
- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio.
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute
- try running the experiment first, then running the live/dead dye through second.

  • Substrates (JZ)

- new set made, in glove box: the 1st 3 on the DOE. SERS signal strong, SEM today

  • DOE for the experiment.

- need to add humidity as it changes even in the glove box

  • Colloid experiment. inconclusive. Look at SEMs

- difficulty in locating the bacteria
- needs much higher particle/bacteria ratio
- experiments outside the channel first?

  • Prospectus framework/discussion: target the end of this week 1/23/09.


† Valve Array/Valve building (FJ)

  • Turn over platform to Teddy
  • TEDDY needs to meet with Mark.


† Fraunhofer: LOAC

  • So so close!


† Biointerfaces group


  • Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week.
  • Made new design for trapper.
  • Mask writer was broken.


† CIMIT- Colson Grant

  • IRB still going/ revisions by tomorrow to Ian. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


† PCR

  • Improve the gel concentration 1.2% agarose. See if you can separate out primer dimers. UPDATE?
  • Look into Melting temp analysis on the 7300 - Done. what is contaminated?
  • Only 1/5 chips are good at the hot embossing step. The vaccum chuck will be checked and o-rings changed - helped a bit. ON HOLD

* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD

  • Try microscope for looking a fluor. Learn how to look at the data in Image J
  • 52, 60, 65 C repeats.


† RCA

  • Cathie submitted One pager Whitepaper. Waiting for response.


† Senior project

  • Megan 1)did some preliminary bonding with Zeonex and teflon film. 2) emailed off the Mask Design to FineLine

† F31: Cochlea

  • Set up adviser meeting Jan
  • Post Gent vs. Pre gent - 4 proteins. 3/4 no change.


† Silica Optimization (Lambda):

  • Update by 12/24.
  • Do three elutions.
  • Next E. coli.


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