Klapperich Lab:Notebook/Lab Meeting Notes/2009/01/08: Difference between revisions
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† Flu R01 <br> | † Flu R01 <br> | ||
* Check on sample amount.<br> | |||
* Plaque Assay. <br> | * Plaque Assay. <br> | ||
* Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09). <br> | * Alex needs to be trained to make SU molds. Trained to make the monoliths (by Hussam By 1/15/09)--we're about to start the training--. <br> | ||
<br> | <br> | ||
† SEPSIS Project <br> | † SEPSIS Project <br> | ||
* Sonali working on first draft of new version paper by | * Sonali working on first draft of new version paper by 1/19. <br> | ||
* Hussam working on Lambda phage data. <br> | * Hussam working on Lambda phage data. <br>. | ||
* 1 ml methanol wash seems to be taking care of the issue. <br> | |||
<br> | <br> | ||
†RNA project | †RNA project | ||
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- tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. <br> | - tests: supernatant fluorescent? excessive dye to observe time-lapse change in green/red ratio. <br> | ||
- omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute <br> | - omit washing step before dilution: wash, dye, incubate, dilute, instead of wash, dye, incubate, wash, dilute <br> | ||
- try running the experiment first, then running the live/dead dye through second. <br> | - '''try running the experiment first, then running the live/dead dye through second.''' '''Bold text'''<br> | ||
- Petridish culture after running evaporation w/o dye in hydrophobic treated channel. <br> | - Petridish culture after running evaporation w/o dye in hydrophobic treated channel. <br> | ||
* Hydrophobic coating of microchannel | * Hydrophobic coating of microchannel | ||
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- needs much higher particle/bacteria ratio <br> | - needs much higher particle/bacteria ratio <br> | ||
- experiments outside the channel first? <br> | - experiments outside the channel first? <br> | ||
* Look at the unused substrates in SEM one week old or more. <br> | |||
* Prospectus framework/discussion: target the end of this week 1/23/09. <br> | * Prospectus framework/discussion: target the end of this week 1/23/09. <br> | ||
<br> | <br> | ||
† Valve Array/Valve building (FJ) <br> | † Valve Array/Valve building (FJ) <br> | ||
* | * Teddy on track. <br> | ||
<br> | <br> | ||
† Fraunhofer: LOAC <br> | † Fraunhofer: LOAC <br> | ||
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† Biointerfaces group <br> | † Biointerfaces group <br> | ||
* <br> | * <br> | ||
* Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. <br> | * Strobe experiments? Jason cannot do them, perhaps MCK can. MCK will talk to Jason this week. Look at the bacteria as they flow. <br> | ||
* | * CR mask was printed new design for trapper . <br> | ||
* | * UV filter installed <br> | ||
* Cathie needs a copy of MCK's, Journal of Applied Physics paper. <br> | |||
* Experimental Data for paper.What do we need in terms of flow rate, seeding densities, etc. <br> | |||
* Do 10 micron simulations. <br> | |||
<br> | <br> | ||
† CIMIT- Colson Grant<br> | † CIMIT- Colson Grant<br> | ||
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<br> | <br> | ||
† Silica Optimization (Lambda): <br> | † Silica Optimization (Lambda): <br> | ||
* | * Elution of C and D total DNA mass 2ng and 0.2 ng [http://openwetware.org/wiki/Image:C_and_D_results.pdf] <br> | ||
* | * Testing with water 12 channels- standard protocol- NA replaced with NF water [http://openwetware.org/wiki/Image:212808-12_samples_water_control_in_channels.pdf] <br> | ||
* 10 channels - 5ch methanol-water / 5ch methanol-buffer-water [http://openwetware.org/wiki/Image:01-08-09_testing_methanol-buffer-water_in_channels.pdf] <br> | |||
* Next E. coli. <br> | * Next E. coli. <br> | ||
Revision as of 14:24, 8 January 2009
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8 January 2009 Lab Meeting Agenda
† Announcements
† COBRA
- problems: dead but green in the end
- RIE (CHF3), Rain?, and Cytop coated and thermal bonding with PTFE filter tested.
- new set made, in glove box: the 1st 3 on the DOE. SEM and SERS available.
- need to add humidity as it changes even in the glove box - this is no longer an issue, asked Ranjith and now can adjust humidity to constant
- difficulty in locating the bacteria
† Valve Array/Valve building (FJ)
* Fixuture for PCR chip. The heater and the thermocouples would be on the fixture. ON HOLD
† F31: Cochlea
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